The industrial localization has witnessed a significant evolution and an important development in the Great Tunis agglomeration, even huge implications and connections with its urban space. In fact, industrial activities which have beenincreasing more than thirty years ago, have brought up new needs and necessities for spatial organization and management structures for their implementation. Nevertheless, the studies and research works on the relationship between localization of industries and urban challenges remain rare. For this reason, we have chosen to concentrate our effort on the analysis of the binomial industrialization-urbanization, two concomitant phenomena marked sometimes by solidarity, sometimes by rupture. More precisely, we intend to contribute through this research, to the identification andthe understanding of industrial activities’ localization and to establish the principal characteristics in connection with the urban development of Great Tunis agglomeration and the recent industrial trend. They are not the industrial activitiesthemselves, considered in an abstract way, that firstly interest us, but their materialization via the industrial zones where they seem to be more present. We will contribute to a spatial analysis of the cross issue gathering industry and urbanizationand particularly the link between industrial localization and the urban development at stake.