The Fermi Gamma-ray Space Telescope was launched on 2008 June 11, carrying the Large Area Telescope (LAT), sensitive to gamma-rays from 20 MeV to more than 300 GeV. Its constantly improving sensitivity and performance offer a new opportunity to understand the sources of the gamma-ray sky including Pulsar Wind Nebulae (PWNe). PWNe are powered by the constant injection of a relativistic wind of electrons and positrons from their central pulsars. These charged particles are accelerated at the shock front forming the PWN and emit photons which can be observed along the entire electromagnetic spectrum, including the highenergy gamma-ray domain via inverse Compton scattering.This thesis present the detailed analysis of two sources of gamma-ray emission potentially asso4021ciated to PWNe : MSH 11-62 and HESS J1857+026. The combination of the spatial and spectral analyses provide new elements to confirm these associations.In a second step, we describe a search for counterparts to sources detected by Cerenkov telescopes.This search led to the detection of six new LAT sources potentially associated with PWNe. These studies bring new insights and constraints on the physical properties of the sources as well as on emitting processes by constraining the models and allowing population studies.