This thesis addresses the issues of coherent distributed knowledge production and sharing in the Life-science area. In spite of the continuously increasing computing and storage capabilities of computing infrastructures, the management of massive scientific data through centralized approaches became inappropriate, for several reasons: (i) they do not guarantee the autonomy property of data providers, constrained, for either ethical or legal concerns, to keep the control over the data they host, (ii) they do not scale and adapt to the massive scientific data produced through e-Science platforms. In the context of the NeuroLOG and VIP Life-science collaborative platforms, we address on one hand, distribution and heterogeneity issues underlying, possibly sensitive, resource sharing ; and on the other hand, automated knowledge production through the usage of these e-Science platforms, to ease the exploitation of the massively produced scientific data. We rely on an ontological approach for knowledge modeling and propose, based on Semantic Web technologies, to (i) extend these platforms with efficient, static and dynamic, transparent federated semantic querying strategies, and (ii) to extend their data processing environment, from both provenance information captured at run-time and domain-specific inference rules, to automate the semantic annotation of ``in silico'' experiment results. The results of this thesis have been evaluated on the Grid'5000 distributed and controlled infrastructure. They contribute to addressing three of the main challenging issues faced in the area of computational science platforms through (i) a model for secured collaborations and a distributed access control strategy allowing for the setup of multi-centric studies while still considering competitive activities, (ii) semantic experiment summaries, meaningful from the end-user perspective, aimed at easing the navigation into massive scientific data resulting from large-scale experimental campaigns, and (iii) efficient distributed querying and reasoning strategies, relying on Semantic Web standards, aimed at sharing capitalized knowledge and providing connectivity towards the Web of Linked Data.
