Tackling challenging systems by biomolecular NMR spectroscopy: Molecular kinetics by fast NMR and bacterial cell wall by solid-state NMR

Standard multidimensional NMR methods required to yield sufficient atom- resolution are time-consuming rendering real-time analysis difficult. The first part of this thesis treats recent advances in the field of fast NMR. The first part of this thesis is concerned with the optimization and extension of longitudinal relaxation enhanced pulse sequences in the context of SOFAST and BEST type experiments. The second part of this thesis deals with the bacterial cell wall of Gram-negative and Gram-positive bacteria. Because of its size and non cristalline character we applied solid state NMR to study it. The high-quality solid-state NMR spectra allow atom-resolved investigation of structure and dynamics of the peptidoglycan and its covalentely bound teichoic acids (WTA). The determination of dynamical properties of the peptidoglycan can also be used to study peptidoglycan-protein interactions and complexation with divalent ions.