Stereoscopic-3DTV (S-3DTV) should provide enhanced depth perception to viewer while it might affect other factors of user experience. Subjective assessment is the most direct way to assess quality of experience (QoE). However, conventional assessment methods are not sufficient to evaluate the QoE of S-3DTV. This thesis aims first to propose new methodologies to evaluate S-3DTV QoE; second, investigate different technical issues related to QoE along the 3DTV broadcasting chain; third, propose recommendations to optimize the S-3DTV QoE. For methodological aspects, the key idea relies on using multidimensional QoE indicators. Visual fatigue, as a particular dimension of QoE, is addressed separately under optimized viewing conditions using questionnaire, vision test and EEG signals. For other QoE indicators, we design subjective QoE experiments to investigate the impact of content acquisition, 3D representation format, compression and transmission on QoE of S-3DTV. The experiment results quantitatively reveal how perceived binocular depth, compression distortion, the cooperation between 3D representation formats and line interleaved display, and view asymmetries affect multidimensional QoE of S-3DTV. Additionally, we elaborate and validate improved stereoscopic shooting rules, depth budget for visual comfort, appropriate frame compatible format for line interleaved display, bitrate to broadcast S-3DTV, threshold for view asymmetries to avoid visual discomfort.