This thesis proposes a flexible approach for integrating industrial applications that is named ODSOI (Ontology-Driven Service-Oriented Integration). Our approach focuses on three complementary problematics that are respectively: the problematic of building of the architecture of enterprise services (PSyn), the problematic of building of the semantic architecture (PSem), and the problematic of building of the integration architecture (PInt). Our approach is based on three major principles that are openness, unification and urbanization. First, the openness principle imposes us to use and to conform to industrial standards. Second, the unification principle allows to make information system components uniform. Third, the urbanization principle allows to correctly structuring the service architecture, the semantic architecture and also the integration architecture. Basing on these three complementary architectures, we propose an implementation of a prototype and also various experimentations that we led within an industrial enterprise.