4He and 3He are model systems for understanding quantum properties of strongly interacting matter. For this reason many studies have been devoted for the understanding of their dynamics. At low temperatures at which quantum effects play an essential role, the elementary excitations in 4He are described by a phonon-roton collective mode. For 3He, the physical description is more complicated, the spectrum has two components: collective excitations (zero-sound) and incoherent particle-hole excitations. Both are described by Landau's theory of Fermi liquids which is valid at low wave vectors. So far, it was thus believed that the dynamics at high wave vectors is essentially incoherent. This thesis is mainly concerned by exploring the collective excitations of a two dimensional 3He film adsorbed on graphite, using inelastic neutron scattering. Such an experiment has three main requirements: a dilution refrigerator in order to work at low temperatures, a time of flight spectrometer for measuring the dynamical structure factor of 3He and a solid substrate (exfoliated graphite ZYX) to obtain a two dimensional film by physical adsorption. Our investigations of the dynamics in two-dimensional 3He adsorbed on graphite preplated with 4He films have revealed important features: At low wave-vectors, the zero-sound mode is considerably depressed compared to bulk 3He. At higher wave vectors, the collective excitations branch enters the particle-hole continuum, and reappears at the lower energy branch of the continuum. This new branch, observed for the first time, is described by the dynamic many-body theory developed by our collaborators from Johannes Kepler University, Linz, Austria. During this work several low temperature techniques have been developed, in particular a robust, cryogen-free dilution refrigerator adapted to the demanding conditions of a neutron scattering experiments. Due to its efficient design, the cooling time has been considerably reduced compared to that of refrigerators of the same type developed in the past.