This study proposes an integrated analysis of molecular biomarkers of landuse, from their genesis in soils to their archiving in the lacustrine sediment. An inventory of neutral lipids is realized on soils of two contrasted landuses: grassland/ pasture and forest. This approach globally allows to distinguish (1) ubiquist linear compounds and (2) (poly-)cyclic compounds, generally speci c. Among these ones, triterpenyl acetates and methoxyserratenes are detected and are respectively biomarkers of Asteraceae and Pinaceae. The persistence of a molecular imprint of an ancient landuse is also demonstrated. Then, the multi-proxy analysis of a sedimentary core, covering the last 6700 years, shows the prominent impact of human activities on the hydrologic functioning of the lake since the gallo-roman period. Molecular assemblages previously de ned in soils are globally detected in sediments, associated with a new speci c biomarker of hemp, of palaeoenvironmental interest. Hypotheses for the reconstitution of past landuses appear to be consistent with previous historical and palaeoenvironmental data.
