Deregulation of alternative splicing during HTLV-1 infection : role of Tax
type: Dataset
description: Reprogramming cellular gene transcription sustains HTLV-1 viral persistence that ultimately leads to the development of adult T-cell leukemia/lymphoma (ATLL). We hypothesized that besides these quantitative transcriptional effects. HTLV-1 quantitatively modifies the pattern of cellular gene expression. Exon expression analysis shows that patients’ untransformed and malignant HTLV-1+ CD4+ T-cells exhibit multiple alternate exon usage (AEU) events. These affect either transcriptionally modified or unmodified genes, culminate in ATLL, and unveil new functional pathways involved in cancer and cell cycle. A total of 486 exon modifications occurred in untransformed infected CD4+ cells were detected in ATLL arguing for a role of AEUs in HTLV-1 leukemogenesis. Unsupervised hierarchical clustering of array data permitted to isolate exon expression patters of 3977 exons that discriminate uninfected, infected, and transformed CD4+ T-cells. Exposing cells to splicing inhibitors revealed that Sudemycin E reduces cell viability of HTLV-1 transformed cells without affecting primary control CD4+ cells and HTLV-1 negative cell lines, suggesting that the huge excess of AEU might provides news targets for treating ATLL. Taken together, these data reveal that HTLV-1 significantly modifies the structure of cellular transcripts and unmask new putative leukemogenic pathways and possible therapeutic targets
landing page: https://theses.hal.science/tel-00995617
identifier: NNT: 2014LYO10059
keyword: alternative-splicing
keyword: arn-polymerase
keyword: epissage-alternatif
keyword: htlv-1
keyword: infoeu-reposemanticsdoctoralthesis
keyword: nf-kb
keyword: rna-polymerase-ii
keyword: sdvsalife-sciences-q-bioagricultural-sciences
keyword: tax
keyword: theses
issued: 2026-05-05T10:22:45.885226
modified: 2026-05-05T10:22:45.885230
language: fr
contact point: Organization
  fn: CCSD

test_moissonnage_selune
type: Agent

Deregulation of alternative splicing during HTLV-1 infection : role of Tax
type: Distribution
format: HTML
access URL: https://theses.hal.science/tel-00995617
issued: 2026-05-05T10:22:45.889868
modified: 2026-05-05T10:22:45.873665