AMH signaling pathway and its target genes in female reproductive tract
type: Dataset
description: Anti-Müllerian hormone (AMH) is a member of the TGF-ß superfamily. AMH is well known for its role in Müllerian duct regression in male fetuses. Postnatally, AMH is secreted by granulosa cells (GCs) of small growing follicles (preantral and small antral). However, despite the increasing interest of ovarian AMH in clinics, little is known on its mechanism of action and its role in female reproductive tract. My PhD project focuses on the identification of AMH function in the female reproductive tract.AMH signals through a type II transmembrane serine/threonine kinase receptor (AMHR-II) which forms a complex with a type I serine/threonine kinase receptor (ActR-IA, BMPR-IA, BMPR-IB). The type II receptor phosphorylates serine and threonine residues of type I receptor. Once activated, the type I receptor phosphorylates the receptor-regulated Smads (R-Smad1/5/8) which interact with a common partner Smad4. The Smad complex accumulates into the nucleus and regulates target gene expression. This canonical signalling pathway is regulated at different levels, in particular by co-receptors which amplify or antagonize TGF-ß family members action. The type I receptors and R-Smads involved in AMH effects on post-natal GCs remain unknown. In addition, to date, no co-receptor has been found for AMH. To define the involvement of the different type I receptors, we used siRNA technology to inactivate Acvr1, Bmpr1a and Bmpr1b in GC. In parallele, we analysed GC extracted from conditional mutant mice for Acvr1 and Bmpr1a. We found that BMPR-IA is the most important type I receptor for AMH to transduce its signal in GC. A Smad-Gal4/UAS-luciferase reporter gene technology allowed us to show that Smad1 and 5 are involved in AMH signaling pathway. Recently, new BMPs coreceptors were found, RGMs for Repulsive Guidance Molecules. There are three RGMs : RGMa, b and c. Because AMH shares with BMPs its type I receptors and R-Smad proteins, we hypothesized that they also share the same co-receptors…
identifier: NNT: 2014PA11T014
issued: 2026-05-05T11:33:50.480988
language: fr
modified: 2026-05-05T11:33:50.480995
contact point: Organization
  fn: CCSD
keyword: anti-mullerian-hormone
keyword: cellules-de-la-granulosa
keyword: genes-cibles
keyword: granulosa-cells
keyword: hormone-anti-mullerienne
keyword: infoeu-reposemanticsdoctoralthesis
keyword: ovaire
keyword: ovary
keyword: sdvsalife-sciences-q-bioagricultural-sciences
keyword: signalisation
keyword: target-genes
keyword: theses
keyword: uterus
landing page: https://theses.hal.science/tel-00989662

AMH signaling pathway and its target genes in female reproductive tract
type: Distribution
format: HTML
issued: 2026-05-05T11:33:50.497826
modified: 2026-05-05T11:33:50.432798
access URL: https://theses.hal.science/tel-00989662

test_moissonnage_selune
type: Agent