Développement d'un biocapteur couplant la résonance des plasmons de surface et la microcalorimétrie pour le suivi des interactions moléculaires à l'interface liquide/solide
type: Distribution
access URL: https://theses.hal.science/tel-00982122
format: HTML
issued: 2026-05-05T13:37:49.699672
modified: 2026-05-05T13:37:49.682999

test_moissonnage_selune
type: Agent

Développement d'un biocapteur couplant la résonance des plasmons de surface et la microcalorimétrie pour le suivi des interactions moléculaires à l'interface liquide/solide
type: Dataset
contact point: Organization
  fn: CCSD
keyword: ondes-de-surface
keyword: resonance-des-plasmons-de-surface-spr
keyword: chimie-de-surface
keyword: microelectronic
keyword: surface-plasmon-resonance-spr
keyword: surface-chemistry
keyword: theses
keyword: surface-acoustic-wave-saw
keyword: spiotherengineering-sciences-physicsother
keyword: infoeu-reposemanticsdoctoralthesis
keyword: microcalorimetrie
keyword: microfabrication
landing page: https://theses.hal.science/tel-00982122
description: To begin with, the characteristics of a DNA hybridization intermolecular interaction at the interface were defined in order to deduce the specifications for our transducers. Following this, the SPR and microcalorimetric transducer will be design by taking into account the constraints of each one. Following the theoretical design of the sensor, a manufacturing process compatible with standard methods of microelectronics manufacturing was tested and identified. To ensure the functionality of the devices, a test of surface functionalization on the sensor was applied to test the compatibility of the manufacturing process with the surface functionalization methods. Finally, a system of active mixing was tested and characterized with a microcalorimetric device to ensure it was possible to mix fluids with organic products to be sure of the quality of the surface reaction. The system developed can be used to mesure DNA hybridization at the interface. This system incorporates two modalities for real-time characterization of intermolecular interactions near the solid/liquid interface. This type of system allows the kinetic measurement of different biological models such as cellular receptors or it is possible to have some molecular measure conformation near the interface. Oxidation of glucose with the enzyme glucose oxidase was shown to present the possibility to measure a biological interaction.
identifier: NNT: 2013ECDL0040
issued: 2026-05-05T13:37:49.695458
language: fr
modified: 2026-05-05T13:37:49.695464