Imaging the neuronal activity from the whole brain of a zebrafish using selective plane illumination microscopy
type: Dataset
description: One third of the manuscript deals with the design of a set-up to deliver tactile stimulation to a human subject. Using microneurography, we recorded the neural response of the mecanoreceptors during a controlled stimulation. The set-up provides precise control over the position, the orientation, the speed and the loading force of a stimulus that we machined on a micro-mill. Thus we can study the transduction of the information, from initially contained in the surface geometry up to the neural coding. The second part shows the construction of Single Plane Illumination Microscope designed to record the neuronal activity of a zebrafish larva. We aim at recording the whole chain of transmission from the sensor to the brain. The fish are genetically modified to express the GCaMP3 calcium indicator in each of their neurons. With the SPIM, where the excitation light comes from the side of the specimen in the shape of a lightsheet and the observation is made from the top at a 90 degrees angle, we can increase the frame rate and the size of the field of view simultaneously, compared to usual techniques (confocal or 2-photons microscopes). These improvements are used to study the correlations between signals from neurons distributed all over the larva?s brain, and discover the underlying networks.
identifier: NNT: 2014PA066032
issued: 2026-05-05T14:27:06.437389
language: fr
modified: 2026-05-05T14:27:06.437396
contact point: Organization
  fn: CCSD
keyword: calcium-imaging
keyword: imagerie-calcique
keyword: infoeu-reposemanticsdoctoralthesis
keyword: laser-sheet
keyword: mecanorecepteurs
keyword: mecanoreceptors
keyword: microneurographie
keyword: microneurography
keyword: nappe-laser
keyword: poisson-zebre
keyword: sdvbbmbplife-sciences-q-biobiochemistry-molecular-biologybiophysics
keyword: theses
keyword: transduction
keyword: zebrafish
landing page: https://theses.hal.science/tel-00979762

Imaging the neuronal activity from the whole brain of a zebrafish using selective plane illumination microscopy
type: Distribution
format: HTML
issued: 2026-05-05T14:27:06.448687
modified: 2026-05-05T14:27:06.407499
access URL: https://theses.hal.science/tel-00979762

test_moissonnage_selune
type: Agent