Role of chemokine receptor CX3CR1 in the monocytes mobilization induced by chemotherapy
type: Dataset
description: Chemokines orchestrate immune response especially by leucocytes mobilization. Myeloidcells, notably monocytes, are involved in inflammation and cancer development. Indeedmonocyte-derived cells and macrophages are strongly represented in tumourmicroenvironment and are associated with a bad prognosis. Characterization of mechanismsleading to monocytes recruitment within the tumor is thus a major issue in anti-cancertherapeutic protocols optimization. Based on the differential expression of chemokinereceptors CCR2 and CX3CR1, two populations of monocytes, inflammatory monocytes(CCR2+, CX3CR1low) and resident monocytes (CCR2-, CX3CR1high) have been characterized inmice which are involved in monocytes recruitment and differentiation into macrophages.The main objective of my work was to better understand the mechanisms of monocytesmobilization following chemotherapeutic treatment. I started to study the role of chemokinereceptors with a focus on CX3CR1 in monocytes reconstitution following cyclophosphamide(CP) treatment. CP is an alkylant agent known for its myelosuppressive properties. Thischemotherapeutic agent induces a transitory anti tumour immune response associated witha monocyte renewal and a strong infiltration of the tumour by adoptively transferred Tlymphocytes. However, antigen-specific T cells are trapped by tumour-associated dendriticcells (TuDCs). This potentially decreases interactions between T lymphocytes and tumourcells suggesting an immunosuppressive role of TuDCs. CP induces a strong depletion ofmyeloid cells followed by a massive reconstitution of bone marrow and spleen monocytesreservoirs. CX3CR1 expression on bone marrow monocytes is decreased duringreconstitution and correlated with a decreasing adhesion of these cells to CX3CL1 ex vivo.We highlighted an increased mobilization of inflammatory monocytes in CX3CR1-/- micecompared to WT and CCR2-/-. Intra vital imaging of bone marrow within CX3CR1-/- mice orwith the help of a CX3CR1 antagonist allowe…
identifier: NNT: 2013PA11T008
issued: 2026-05-06T01:17:14.070475
language: fr
modified: 2026-05-06T01:17:14.070480
contact point: Organization
  fn: CCSD
keyword: bone-marrow
keyword: chemokine
keyword: chemotherapy
keyword: chimiokine
keyword: chimiotherapie
keyword: cx3cr1
keyword: infoeu-reposemanticsdoctoralthesis
keyword: moelle-osseuse
keyword: monocyte
keyword: monocytes
keyword: sdvimmlife-sciences-q-bioimmunology
keyword: theses
landing page: https://theses.hal.science/tel-00959111

Role of chemokine receptor CX3CR1 in the monocytes mobilization induced by chemotherapy
type: Distribution
format: HTML
issued: 2026-05-06T01:17:14.091401
modified: 2026-05-06T01:17:14.051261
access URL: https://theses.hal.science/tel-00959111

test_moissonnage_selune
type: Agent