@prefix dcat: . @prefix dct: . @prefix foaf: . @prefix vcard: . @prefix xsd: . a dcat:Dataset ; dct:description """ Chemokines orchestrate immune response especially by leucocytes mobilization. Myeloidcells, notably monocytes, are involved in inflammation and cancer development. Indeedmonocyte-derived cells and macrophages are strongly represented in tumourmicroenvironment and are associated with a bad prognosis. Characterization of mechanismsleading to monocytes recruitment within the tumor is thus a major issue in anti-cancertherapeutic protocols optimization. Based on the differential expression of chemokinereceptors CCR2 and CX3CR1, two populations of monocytes, inflammatory monocytes(CCR2+, CX3CR1low) and resident monocytes (CCR2-, CX3CR1high) have been characterized inmice which are involved in monocytes recruitment and differentiation into macrophages.The main objective of my work was to better understand the mechanisms of monocytesmobilization following chemotherapeutic treatment. I started to study the role of chemokinereceptors with a focus on CX3CR1 in monocytes reconstitution following cyclophosphamide(CP) treatment. CP is an alkylant agent known for its myelosuppressive properties. Thischemotherapeutic agent induces a transitory anti tumour immune response associated witha monocyte renewal and a strong infiltration of the tumour by adoptively transferred Tlymphocytes. However, antigen-specific T cells are trapped by tumour-associated dendriticcells (TuDCs). This potentially decreases interactions between T lymphocytes and tumourcells suggesting an immunosuppressive role of TuDCs. CP induces a strong depletion ofmyeloid cells followed by a massive reconstitution of bone marrow and spleen monocytesreservoirs. CX3CR1 expression on bone marrow monocytes is decreased duringreconstitution and correlated with a decreasing adhesion of these cells to CX3CL1 ex vivo.We highlighted an increased mobilization of inflammatory monocytes in CX3CR1-/- micecompared to WT and CCR2-/-. Intra vital imaging of bone marrow within CX3CR1-/- mice orwith the help of a CX3CR1 antagonist allowed us to show a specific role of CX3CR1 in thelumen crawling and confinement of monocyte-derived cells in both sinusoid andparenchyma of the bone marrow. We suggest that CX3CR1 controls the release of bonemarrow monocytes during CP-induced mobilization. We think that modulating the rate ofcellular mobilization, by increasing the host’s leukocyte pool during CP inducedreconstitution and/or targeting CX3CR1, could contribute to improve cellular responsefollowing tissue damage or immune cell dysfunction. Furthermore, targeting CX3CR1couldprovide applications in the stem cell transplantation domain. """ ; dct:identifier "NNT: 2013PA11T008" ; dct:issued "2026-05-06T01:17:14.070475"^^xsd:dateTime ; dct:language "fr" ; dct:modified "2026-05-06T01:17:14.070480"^^xsd:dateTime ; dct:publisher ; dct:title "Role of chemokine receptor CX3CR1 in the monocytes mobilization induced by chemotherapy" ; dcat:contactPoint [ a vcard:Organization ; vcard:fn "CCSD" ] ; dcat:distribution ; dcat:keyword "bone-marrow", "chemokine", "chemotherapy", "chimiokine", "chimiotherapie", "cx3cr1", "infoeu-reposemanticsdoctoralthesis", "moelle-osseuse", "monocyte", "monocytes", "sdvimmlife-sciences-q-bioimmunology", "theses" ; dcat:landingPage . a dcat:Distribution ; dct:format "HTML" ; dct:issued "2026-05-06T01:17:14.091401"^^xsd:dateTime ; dct:modified "2026-05-06T01:17:14.051261"^^xsd:dateTime ; dct:title "Role of chemokine receptor CX3CR1 in the monocytes mobilization induced by chemotherapy" ; dcat:accessURL . a foaf:Agent ; foaf:name "test_moissonnage_selune" . a foaf:Document .