Electric field-cell interaction : conception of microfluidic biochips for cell pairing and fusion by electric field pulses
type: Dataset
description: Cell fusion is a method to generate a hybrid cell combing the specific properties of its progenitor cells. Initially developed for antibody production, it is now also investigated for cancer immunotherapy. Electrofusion consists on the production of hybridoma using electric pulses. Compared to viral or chemical methods, electrofusion shows higher yields and this system is contaminant free. Actually, electrofusion is investigated in electroporation cuvettes, where the electric field is not precisely controllable and cell placement impossible, resulting in low binuclear hibridoma yields. To improve the fusion quality and yield, cell capture and pairing are necessary.Our objective was the development and realization of biochips involving microelectrodes and microfluidic channels to place and pair cells prior to electrofusion. A first trapping structure based on insulators and the use of dielectrophoresis has been achieved. In order to perform fluidic experiments, a biocompatible irreversible packaging was developed. Then, the experimental medium was optimized for electrofusion. Confronting the biological experiments and the numerical simulations, we showed that the application of electric pulses leads to a decrease of the cytoplasmic conductivity. The microstructure was validated by cell electrofusion. A yield of 55%, with a membrane fusion duration of 6 s has been achieved. Secondly, we proposed two trapping microstructures for high density electrofusions. The first one is based on a fluidic trapping while the second one uses dielectrophoresis, free of electric wiring, thanks to conductive pads. Up to 75% of paired cells were successfully electrofused with the conductive pads. More than 97% of the hybridoma were binuclear. The trapping being reversible, the hybridoma can be collected for further analysis.
identifier: NNT: 2013PA112286
issued: 2026-05-07T14:02:41.664600
language: fr
modified: 2026-05-07T14:02:41.664607
contact point: Organization
  fn: CCSD
keyword: dielectrophorese
keyword: dielectrophoresis
keyword: electrofusion
keyword: infoeu-reposemanticsdoctoralthesis
keyword: lab-on-chip
keyword: laboratoire-sur-puce
keyword: microfluidics
keyword: microfluidique
keyword: physcondcm-genphysics-physicscondensed-matter-cond-matother-cond-matother
keyword: theses
landing page: https://theses.hal.science/tel-00926219

Electric field-cell interaction : conception of microfluidic biochips for cell pairing and fusion by electric field pulses
type: Distribution
format: HTML
issued: 2026-05-07T14:02:41.666565
modified: 2026-05-07T14:02:41.647268
access URL: https://theses.hal.science/tel-00926219

test_moissonnage_selune
type: Agent