Isolation and characterization of new species of Torque Teno Mini Virus (TTMV) : potential implication in the pathogenesis of pneumonia
type: Dataset
description: Pneumonia is the leading cause of death in children worldwide. It can be caused by a number of known pathogens, but 15-35% of childhood pneumonia are still not associated with an etiologic agent. A pathogen discovery assay allowed us to identify new species of Torque Teno Mini Virus (TTMV, Anelloviridae), named TTMV-LY, in three undiagnosed pleural effusions from children hospitalized with parapneumonic empyema. TTMV are ubiquitous orphan viruses, and their involvement in pathogenesis remains unknown. The respiratory tract was previously described to be a site of anellovirus detection. We investigated the role of these new species in the pathogenesis of severe pneumonia.Full-length TTMV-LY genomes were isolated and in vitro replicated. The response of alveolar epithelial cells, and antigen presenting cells (APC), both involved in the inflammation process, was studied after infection with neo-synthesized virions.This study showed for the first time that: i) TTMV-LY can deeply colonize lungs, ii) alveolar epithelial cells are permissive to the TTMV-LY and allow an efficient replication, iii) viral infection modulates cellular and innate immune responses of alveolar epithelial cells, by inducing gene expression deregulations and inflammatory mediators production, iv) TTMV-LY are able to interact with APC and thereby regulate differentially their inflammatory process.All these results allowed to highlight a potential involvement of TTMV-LY in the pathogenesis of severe pneumonia and brought out the complexity of the biological mechanisms taking place during infection by viruses of this family.
identifier: NNT: 2013ENSL0809
issued: 2026-05-07T15:54:15.144886
language: fr
modified: 2026-05-07T15:54:15.144893
contact point: Organization
  fn: CCSD
keyword: deregulation-de-lexpression-genique
keyword: epanchement-pleural
keyword: gene-expression-deregulation
keyword: infoeu-reposemanticsdoctoralthesis
keyword: innate-immune-response
keyword: mediateurs-inflammatoires-solubles
keyword: parapneumonic-effusion
keyword: phylogenie
keyword: phylogeny
keyword: pneumonia
keyword: pneumonie
keyword: replication-virale
keyword: reponse-immunitaire-innee
keyword: sdvbclife-sciences-q-biocellular-biology
keyword: sdvmhepmilife-sciences-q-biohuman-health-and-pathologyinfectious-diseases
keyword: soluble-inflammatory-mediators
keyword: theses
keyword: ttmv
keyword: viral-replication
landing page: https://theses.hal.science/tel-00923721

Isolation and characterization of new species of Torque Teno Mini Virus (TTMV) : potential implication in the pathogenesis of pneumonia
type: Distribution
format: HTML
issued: 2026-05-07T15:54:15.153602
modified: 2026-05-07T15:54:15.123075
access URL: https://theses.hal.science/tel-00923721

test_moissonnage_selune
type: Agent