Epigenetic regulation of polymerase III RNA transcription machinery by histone deacetylase SIRT1
type: Dataset
description: SIRT1, member of the sirtuins family, is an NAD-dependent deacetylase, playing an essential role in controlling gene expression. In addition to modifying histones, SIRT1 can affect the activity of several transcription factors and their target genes. A fundamental question is to understand the molecular mechanisms by which SIRT1 controls the expression of genesinvolved in cell proliferation and energy metabolism. To identify protein partners of SIRT1, we used the method of TAP-TAG purification from a soluble nuclear fraction and a chromatin anchored fraction of Mef cells stably expressing ectopic copy of SIRT1 (SIRT1-e). We were able to identify a SIRT1 complex associated with both cell proliferation factor Ki67, and TFIIIC,subunit required for assembly of the RNA polymerase III pre-initiation complex. By deleting Sirt1, and by specifically inhibiting Ki67 expression, we showed that the RNA Polymerase III transcription machinery and cell proliferation were strongly affected. All of my results clearly shows that SIRT1, Ki67, and TFIIIC are within a same protein complex, SIRT1 and Ki67, acting in coordination to regulate the expression level of SINES and LINES, transcribed from RNA polymerase III transcription machinery.
identifier: NNT: 2012STRAJ099
issued: 2026-05-07T16:17:57.926440
language: fr
modified: 2026-05-07T16:17:57.926444
contact point: Organization
  fn: CCSD
keyword: infoeu-reposemanticsdoctoralthesis
keyword: ki67
keyword: line
keyword: sdvbbmlife-sciences-q-biobiochemistry-molecular-biology
keyword: sine
keyword: sirt1
keyword: tfiiic
keyword: theses
keyword: transcription
landing page: https://theses.hal.science/tel-00923163

Epigenetic regulation of polymerase III RNA transcription machinery by histone deacetylase SIRT1
type: Distribution
format: HTML
issued: 2026-05-07T16:17:57.932155
modified: 2026-05-07T16:17:57.915969
access URL: https://theses.hal.science/tel-00923163

test_moissonnage_selune
type: Agent