test_moissonnage_selune
type: Agent

Dual role of IFT57 in cilia and nucleus in Paramecium
type: Dataset
contact point: Organization
  fn: CCSD
keyword: paramecium
keyword: ift57hippi
keyword: ift
keyword: sdvsalife-sciences-q-bioagricultural-sciences
keyword: nucleus
keyword: theses
keyword: cil
keyword: infoeu-reposemanticsdoctoralthesis
keyword: noyau
keyword: cilia
landing page: https://theses.hal.science/tel-00922993
description: My thesis work consisted in the study of cilia and flagella, organelles highly conserved in many eukaryotes, which protrude at the cell surface as a microtubular backbone, the axoneme, bounded by an extension of the plasma membrane. A major interest in the study of cilia is that they are involved in the regulation of many cell events, such as re-entry in the cell cycle, and that their dysfunction in vertebrates provokes multi-symptomatic diseases called ciliopathies. Ciliary growth is under control of IFT (intraflagellar transport) mechanism, first discovered in Chlamydomonas. The IFT complex includes at least 17 members and is is composed of two subcomplexes, IFTB linked to kinesin for anterograde transport in ciliary building, and IFTA linked to dynein for retrograde transport in recycling. In my thesis on IFT in Paramecium, I focused on IFT57/Hippi, a member of IFTB, which seems to display two different functions in human cells: biogenesis of cilia as member of the IFTB particle and gene regulation in Huntington’s diseases, as part of a complex with Hip1 that binds caspase promoters involved in apoptosis. Some recent work also showed that in the cytoplasm of non-ciliate cells, IFT57, associated with IFT20 and IFT88, regulates T-cell antigen receptor (TCR) recycling in immune synapse. In Paramecium, four genes issued from two successive genome duplications encode IFT57 (IFT57A – D). The isoforms A and B on the one hand and C and D on the other hand are sufficiently close in DNA sequence to get homology dependent RNAi silencing with only one gene of each pair. I first confirmed that IFT57 Paramecium genes have a conserved IFT function in cilia formation, but apparently not in maintenance, in contrast to other IFT proteins such as IFT46. The combination of RNAi and GFP fusion localization allowed us to suggest interactions between IFT46 and IFT57 in the cytoplasm, upstream from the usual site of interaction in the basal body. I also found that IFT57A-GFP, but not IF…
identifier: NNT: 2013PA112169
issued: 2026-05-07T16:25:13.693435
language: en
modified: 2026-05-07T16:25:13.693441

Dual role of IFT57 in cilia and nucleus in Paramecium
type: Distribution
access URL: https://theses.hal.science/tel-00922993
format: HTML
issued: 2026-05-07T16:25:13.696591
modified: 2026-05-07T16:25:13.679282