Roles and regulation of ion channel ASIC3 in pain
type: Dataset
description: Chronic, inflammatory, neuropathic, or incisional pain is affecting about 20 % of the adult population and up to 50 % of the elderly population. It thus represent a real public health issue. Despite the existence of large families of analgesics, treatments are often ineffective. This is due in large part to a lack of knowledge of the patho-physiological mechanisms of pain. During my PhD, I have been interested in the roles and regulation of molecular sensors of the pain recently highlighted: ion channels (ASICs "Acid Sensing Ion Channels "). ASICs constitute a family of excitatory cationic channels. The ASIC3 channels, in particular, are present in sensory neurons that innervate the skin, muscles, organs and joints. They are activated by low extracellular acidification occurring in many patho-physiological mechanisms such as inflammation, ischemia, tumor growth, or the subsequent tissue damage, for example, surgery. In a first study, we showed that ASIC3 channels play a crucial role in the development of post -operative pain, including postural pain, close to clinical cases. From a plantar incision model in rats, we demonstrated an over-expression of ASIC3 channels in sensory neurons innervating the operated hindpaw. Pharmacological inhibition (with toxin) and invalidation (siRNA) of ASIC3 in vivo reduce pain behavior. Our second study focused on the human ASIC3 channel, not yet extensively studied. I demonstrated that this channel has a unique and inducible property which gives it a sensitivity not only to acidification, but also to the extracellular alkalinization. This alkaline sensitivity is an intrinsic characteristic of the channel. It involves two specific arginine residues in the human channel that are present in its extracellular loop.Thus the human ASIC3 channel adapts its activity at different pH environments, and could participate in the fine regulation of membrane potential and neuronal sensitization. More recently, I have studied the regulation of ASIC…
identifier: NNT: 2013NICE4084
issued: 2026-05-07T19:02:30.025749
language: fr
modified: 2026-05-07T19:02:30.025753
contact point: Organization
  fn: CCSD
keyword: acidification
keyword: alcalinisation
keyword: alkalization
keyword: animal-behavior
keyword: asic3
keyword: comportement-animal
keyword: douleur
keyword: electrophysiologie
keyword: electrophysiology
keyword: inflammatory-lipids
keyword: infoeu-reposemanticsdoctoralthesis
keyword: lipides-inflammatoires
keyword: pain
keyword: sdvsalife-sciences-q-bioagricultural-sciences
keyword: theses
landing page: https://theses.hal.science/tel-00919428

Roles and regulation of ion channel ASIC3 in pain
type: Distribution
format: HTML
issued: 2026-05-07T19:02:30.037020
modified: 2026-05-07T19:02:30.008639
access URL: https://theses.hal.science/tel-00919428

test_moissonnage_selune
type: Agent