Influence of recombinant human erythropoietin on cardiovascular and kidney functions in rats with endothelial dysfunction : effects of interactions with chronic exercise
type: Dataset
description: The chronic administration of rHuEPO can engender side effects. An increase of the hematocritinduced by rHuEPO, by increasing the erythrocytosis, the blood viscosity and the shear stress onvascular surface, can be responsible of arterial high blood pressure and arterial thrombosis. Thepresence of a normal endothelial function and nitric oxide (NO) can counter the noxious effectsof rHuEPO. On these bases, we studied the cardiovascular effects of a chronic administration ofrHuEPO in various frames: within doping field, to trained rats with L-NAME-induced NOdependentendothelial dysfunction and within the framework of a treatment, to chronic kidneydisease (CKD) rats developing endothelial dysfunction caused by the "5/6 nephrectomy". In ourdoped rats, we observed an important mortality (51%). A severe arterial high blood pressuredeveloped in these rats (> 220 mmHg) associated with an impairment of the NO-dependentvasorelaxation (< 60 %). CKD rats also showed an increase in blood pressure and an endothelialdysfunction, in response to acetylcholine in the aorta and in response to a rise in flow in perfusedmesenteric artery. These parameters were improved by exercise. Kidney sections stained withSirius red showed marked fibrosis in CKD rats. Fibrosis, creatinine and albumin were decreasedby exercise alone but were increased in rats from the CKD + EPO + Ex group. NAD(P)H oxidaseactivity and the expression of Nox4, p67phox, and MAPK erk1/2 were increased in CKDrats. Exercise or rHuEPO prevented these increases. However, the NAD(P)H oxidase activityand the expression of MAPK erk1/2 remained high in the kidney of rats from the CKD+EPO+Exgroup. Our data suggest that exercise alone has a protective effect against vascular and renaldysfunction and renal fibrosis. These protective effects are linked to the downregulation of theNADPH oxidase activity and MAPK erk1/2 signaling pathways. However, exercise combinedwith rHuEPO treatment has deleterious effects on kidney structure and fu…
landing page: https://theses.hal.science/tel-00919398
identifier: NNT: 2011AVIG0707
keyword: chronic-kidney-disease
keyword: dopage
keyword: doping
keyword: dysfonction-endotheliale
keyword: endothelial-dysfunction
keyword: erythropoietin
keyword: erythropoietine
keyword: exercice
keyword: exercise
keyword: infoeu-reposemanticsdoctoralthesis
keyword: insuffisance-renale-chronique
keyword: oxidative-stress
keyword: sdvmheplife-sciences-q-biohuman-health-and-pathology
keyword: stress-oxydatif
keyword: theses
issued: 2026-05-07T19:01:12.604000
modified: 2026-05-07T19:01:12.604007
language: fr
contact point: Organization
  fn: CCSD

test_moissonnage_selune
type: Agent

Influence of recombinant human erythropoietin on cardiovascular and kidney functions in rats with endothelial dysfunction : effects of interactions with chronic exercise
type: Distribution
format: HTML
access URL: https://theses.hal.science/tel-00919398
issued: 2026-05-07T19:01:12.608054
modified: 2026-05-07T19:01:12.582965