Identification of a terminal uridylyl transferase implicated in the protection of deadenylated messager RNAs 3' end in Arabidopsis thaliana
type: Dataset
description: The work presented in this manuscript defines a new role of mRNA uridylation, using Arabidopsis as a model organism. RNA uridylation is catalyzed by RNA nucleotidyltransferases belonging to the non canonical poly(A) polymerase (ncPAP) family. Among the 14 genes encoding ncPAPs in Arabidopsis, we identified a terminal uridylyl transferase, TUT1, responsible for mRNA uridylation. Our results show that mRNAs are uridylated by TUT1 after a deadenylation step. Uridylation doesn’t modify mRNA degradation rates but is essential for deadenylated mRNA 3’ end protection against 3’- 5’ exoribonucleolytic attacks and to prevent 3’ truncated aberrant mRNA formation. Interestingly, this protection by uridylation is detected in polysomes. One biological function of mRNA uridylation is to establish a 5’-3’ mRNA degradation polarity that could be essential in the case of cotranslational mRNA decay.
landing page: https://theses.hal.science/tel-00881163
identifier: NNT: 2012STRAJ132
keyword: arabidopsis
keyword: arn-messager
keyword: degradation-des-arn
keyword: infoeu-reposemanticsdoctoralthesis
keyword: messenger-rna
keyword: rna-degradation
keyword: sdvbbmlife-sciences-q-biobiochemistry-molecular-biology
keyword: theses
keyword: uridylation
issued: 2026-05-09T02:46:49.253644
modified: 2026-05-09T02:46:49.253649
language: fr
contact point: Organization
  fn: CCSD

test_moissonnage_selune
type: Agent

Identification of a terminal uridylyl transferase implicated in the protection of deadenylated messager RNAs 3' end in Arabidopsis thaliana
type: Distribution
format: HTML
access URL: https://theses.hal.science/tel-00881163
issued: 2026-05-09T02:46:49.259786
modified: 2026-05-09T02:46:49.243859