Effets des champs électriques pulsés milli et nanosecondes sur cellules et tissus
type: Dataset
description: Electropermeabilization is a physical technique first developed to transfer cytotoxic drugs in tumor. It consists in the transient permeabilization of the plasma membrane following electric field application. In specific electric conditions using long pulses of several milliseconds, the membrane destabilization can allow transferring plasmid DNA into the cell, thus allowing the development of gene therapy. For now, one clinical trial has been published using gene eletro-transfer and several others are ongoing. However the efficiency of the technique remains low compared to other transfer methods. This thesis gets interested in how pulsed electric fields affect cell membranes, in the concrete situation of gene transfer by electroporation. The comprehension of electro-gene transfer process need to be well understood in order to optimize it. In this context, we focus on the 3 barriers that DNA is confronted to during its transfer: first at the cell level: plasma membrane and nuclear envelope, second at the tissue level: the complexity of a multicellular environment. i) We first studied the efficiency of gene transfer on multicellular spheroid model. This work allowed the validation of this model for electro-transfection study, and the further optimization of the technique by raising some of the failures encountered in gene transfer in tissue. ii) The second part of the work has been dedicated to study plasma membrane destabilization due to electroporation by Atomic Force Microscopy. We used both innovating imaging modes and spectroscopy modes to analyze the effects on living cells, which resulted in the measurement of a decrease in elasticity, linked to side effects of electric fields on actin cytoskeleton destabilization. iii) The last part has been dedicated to the effects of nanopulses (nsEP) on both plasma membrane and the second barrier encountered by gene during its transfer, namely nuclear envelope. The effects of these very short (ns) and intense (several kV/cm…
issued: 2026-05-09T08:24:32.019778
language: fr
modified: 2026-05-09T08:24:32.019782
contact point: Organization
  fn: CCSD
keyword: atomic-force-microscopy
keyword: electropermeabilisation
keyword: electropermeabilization
keyword: electroporation
keyword: gene-transfer
keyword: impulsions-nanosecondes
keyword: infoeu-reposemanticsdoctoralthesis
keyword: microscopie-a-force-atomique
keyword: multicellular-spheroids
keyword: nanosecond-pulse-electric-fields
keyword: spheroides-multicellualaire
keyword: spinanoengineering-sciences-physicsmicro-and-nanotechnologiesmicroelectronics
keyword: theses
landing page: https://theses.hal.science/tel-00874040

Effets des champs électriques pulsés milli et nanosecondes sur cellules et tissus
type: Distribution
format: HTML
issued: 2026-05-09T08:24:32.047682
modified: 2026-05-09T08:24:31.995224
access URL: https://theses.hal.science/tel-00874040

test_moissonnage_selune
type: Agent