Chemical study of resinous materials : olibanum, dammar and mastic : application to artistic and archaeological samples
type: Dataset
description: This work is focused on the study of triterpene part of three natural plant resins commercially available, olibanum(frankincense), dammar and mastic, by various analytical techniques (FTIR, HPLC / UV, HPLC / UV / Fluorimetry and GC -MS). Afluorimetric study was conducted on these three resins via grafting reaction of a fluorescent marker (dansyl chloride), whichallowed to detected triterpene molecules by fluorimetry, decreasing their detection threshold and to obtain a specificfluorimetric fingerprint for each studied resin. This protocol has been successfully applied on an archaeological sample (G14)and the resinous material has been identified (the olibanum resin, the species B. frereana). A triterpenic fraction was extractedby various extraction procedure (reflux, Soxhlet and ultrasounds) using three different solvents (methanol, n-hexane, and dlimonene)in order to determine the optimal conditions for the extraction and identification of triterpenic molecules by HPLC /UV. Two reversed phase columns were tested in this work: a classical column RP-18 (Merck) and column «core-shell» Kinetex(Phenomenex) to try to optimize the analysis conditions. Kinetex column has allowed a reduction of analysis time to 73% foranalyses of olibanum and to 70% for analyses of dammar and mastic resin, and this is a very encouraging result. The optimizedprotocol was successfully applied on the archaeological sample G12, which was supposed to contain mastic resin and thishypothesis was confirmed by HPLC/UV and GC-MS analysis. A quantitative study of performance and the relative area of thepeaks were also performed. The extracts were analyzed by HPLC / UV and GC-MS. Concerning GC-MS analysis, samplepreparation was done through formation of TMS derivatives (trimethylsilyl) with the aim of creating a database of TMSderivatives for dammar and mastic resins. The identification of compounds characterized in this work was done according tothe literature, and corresponding tR et UV spectra. D-li…
identifier: NNT: 2012AVIG0245
issued: 2026-05-09T09:54:26.447294
language: fr
modified: 2026-05-09T09:54:26.447299
contact point: Organization
  fn: CCSD
keyword: archaeological-sample
keyword: artistic-sample
keyword: chimothechemical-sciencesother
keyword: clhpuv
keyword: colonne-kinetex
keyword: cpg-sm
keyword: cultural-heritage-of-bosnia-and-herzegovina
keyword: d-limonene
keyword: dammar
keyword: echantillon-archeologique
keyword: echantillon-artistique
keyword: extraction
keyword: fluorimetrie
keyword: fluorimetry
keyword: frankincense
keyword: gc-ms
keyword: hplc--uv
keyword: infoeu-reposemanticsdoctoralthesis
keyword: kinetex-column
keyword: mastic
keyword: natural-plant-resins
keyword: olibanum
keyword: olivan
keyword: patrimoine-culturel-de-bosnie-herzegovine
keyword: reflux
keyword: resines-naturelles-vegetales
keyword: soxhlet
keyword: theses
keyword: triterpenes
keyword: triterpenic-molecules
keyword: ultrasons
keyword: ultrasound
landing page: https://theses.hal.science/tel-00872166

Chemical study of resinous materials : olibanum, dammar and mastic : application to artistic and archaeological samples
type: Distribution
format: HTML
issued: 2026-05-09T09:54:26.450875
modified: 2026-05-09T09:54:26.403024
access URL: https://theses.hal.science/tel-00872166

test_moissonnage_selune
type: Agent