Role of ECM Physical Properties on Force Distribution and Cell Internal Organization
type: Distribution
access URL: https://theses.hal.science/tel-00870404
format: HTML
issued: 2026-05-09T11:20:57.591574
modified: 2026-05-09T11:20:57.556808

Role of ECM Physical Properties on Force Distribution and Cell Internal Organization
type: Dataset
contact point: Organization
  fn: CCSD
keyword: cell
keyword: theses
keyword: physcondcm-genphysics-physicscondensed-matter-cond-matother-cond-matother
keyword: adhesion
keyword: mecanique
keyword: force
keyword: infoeu-reposemanticsdoctoralthesis
keyword: cytoskeleton
keyword: cytosquelette
keyword: matrice
keyword: matrix
keyword: mechanics
keyword: cellule
landing page: https://theses.hal.science/tel-00870404
description: It has grown a great interest among biophysicists that adherent cell senses substrate stiffness and geometry by the process of mechanotransduction. Cells exert force on the extra cellular matrix on which it is subjected to adhere by active mechanism, which involves biomechanical regulatory feedback loop. It is still unclear whether biomechanical,biochemical or geometrical stimuli dominates invivo. Underlying mechanism behind the way cell senses, redistributes and transmits force still needs to elucidate. In the first part we show how geometrical modulation influences traction force and tension distribution in the actin cytoskeleton, and also localization of focal adhesion at single cell level by combining use of Micropatterning and Traction force microscopy technique. We measure cell traction force seeded on different micropattened shapes(like U,arrow and H) coated with protein on 2D soft polyacrilamide gel embedded with nano beads. We show that geometrical cue redistributes traction force locally while projected area designed for a single cell is conserved. we compare cell traction force developed when cells are on a continuous 2D circular array pattern with discrete 3D micropillar array of same stiffness. We also have investigated how forces are varied with rigidity modulation of the extra cellular matrix in both these two cases. A quantitative measurement has been done on the spatially localized adhesion proteins on the circular dots and also actin re-organization. In the sencod part to achieve more systematic understanding of force distribution we have consider more on force localization and orientation on different patterned geometry( V, T, Tripod, Plus). We correlate force distribution with stress fiber and focal adhesion localization. Finally we look into centrosome distribution in correlation with force and other internal organization. An alternate approach has been made towards the development of thermoresponsive micropattern, made of poly(N-isopropyla cryl…
identifier: NNT: 2012GRENY074
issued: 2026-05-09T11:20:57.572315
language: fr
modified: 2026-05-09T11:20:57.572320

test_moissonnage_selune
type: Agent