Biochemical and structural analysis of multiple interactions of the oncoprotein E6 produced by papillomavirus
type: Dataset
description: The oncoprotein E6 which plays a crucial role in the process of carcinogenesis induced by HPV, has withstood all tests for a long time. Since 1995 the team « oncoproteins » has focused on this issue. This allowed the resolution of structure of C-terminal domain of E6 by NMR analysis, in 2006. In this context, i started my PhD in 2008 with aim to continue the pursuit of structural data on E6 while also acquiring information about its modes of interaction with its cellular targets. The work of this thesis has enabled us to obtain the crystal structure of E6 (HPV16) in complex with a peptide of E6AP, using an original approach capable of producing stable and soluble proteins E6. This structure represents the first structural information on full-length E6, awaited for over 20 years by the scientific community. I also performed during this thesis an analysis of the interaction system of the E6 protein based on a large study of interaction between proteins E6 (7 types) and 93 peptides bearing LxxLL motif.
identifier: NNT: 2012STRAJ098
issued: 2026-05-09T14:00:59.006581
language: fr
modified: 2026-05-09T14:00:59.006585
contact point: Organization
  fn: CCSD
keyword: brd4-protein
keyword: cancer-du-col-de-luterus
keyword: cervial-cancer
keyword: cristallographie
keyword: crystallography
keyword: e2-protein
keyword: e6-oncoproteines
keyword: infoeu-reposemanticsdoctoralthesis
keyword: interaction-proteique
keyword: oncoproteines-e6
keyword: optimisation-de-production-de-proteines
keyword: optimization-of-protein-production
keyword: papillomavirus
keyword: protein-interactions
keyword: proteine-brd4
keyword: proteine-e2
keyword: sdvbbmbslife-sciences-q-biobiochemistry-molecular-biologystructural-biology-q-biobm
keyword: theses
landing page: https://theses.hal.science/tel-00866972

Biochemical and structural analysis of multiple interactions of the oncoprotein E6 produced by papillomavirus
type: Distribution
format: HTML
issued: 2026-05-09T14:00:59.015224
modified: 2026-05-09T14:00:58.988571
access URL: https://theses.hal.science/tel-00866972

test_moissonnage_selune
type: Agent