Two-photon chromophore-polymer conjugates grafted onto gold nanoparticles as fluorescent probes for bioimaging and photodynamic therapy applications
type: Dataset
description: Photodynamic therapy (PDT) is an alternative treatment of cancer requiring the use of chromophore molecules (photosensitizers), which can induce cell death after light excitation. Gold nanoparticles (AuNP), exhibiting localized Surface Plasmon Resonance, can enhance the photophysical response of chromophores located in their vicinity, and thus improve their therapeutic action. Moreover, the use of highly localized two-photon chromophores (photosensitizers and fluorophores), capable to undergo a localized excitation by light in the Near InfraRed region, should increase the penetration depth into tissues, thus improve the treatment efficiency (by PDT) and the imaging (by fluorescence microscopy) of cancer tissues.In this work, we describe the elaboration of water-soluble hybrid nano-objects for PDT and fluorescence bioimaging applications, composed of two-photon chromophore-polymer conjugates grafted onto gold nanoparticles. In order to obtain these nano-objects we follow a multistep strategy: i) the synthesis of a well-defined water-soluble chromophore-polymer conjugates; ii) the end-group oriented grafting of chromophore-polymer conjugates onto 20 nm AuNP. The coupling of hydrophobic two-photon chromophores on linear water-soluble copolymer chains (poly(N-acryloylmorpholine-co-N-acryloxysuccinimide)), obtained by controlled/living RAFT polymerization, resulted in well-defined water-soluble chromophore-polymer conjugates, with different polymer lengths (2 000 g.mol-1 < Mn < 37 000 g.mol-1) and architectures (random or block), and a controlled number of chromophores per chain (varying between 1 and 21). Their grafting onto 20 nm AuNP gave water-soluble hybrid nano-objects with high grafting densities (~0.5 chains/nm²). The role of the polymer chain being to tune the distance between chromophores and AuNP surface, we have evidenced the increase in the polymer corona thickness of grafted AuNP (estimated by TEM) with the increasing polymer Mn, corroborating with the corr…
identifier: NNT: 2012ISAL0125
issued: 2026-05-09T16:39:01.588448
language: en
modified: 2026-05-09T16:39:01.588452
contact point: Organization
  fn: CCSD
keyword: effet-plasmon
keyword: enhanced-fluorescence
keyword: exaltation-de-fluorescence
keyword: gold-nanoparticle
keyword: imagerie-medicale
keyword: infoeu-reposemanticsdoctoralthesis
keyword: medical-imaging
keyword: microscopie-a-deux-photons
keyword: nanoparticule-dor
keyword: photodynamic-therapy
keyword: photoinduced-death
keyword: phototherapie
keyword: phototherapy
keyword: polymerisation-raft
keyword: raft-polymerization
keyword: spiotherengineering-sciences-physicsother
keyword: therapie-photodynamique
keyword: theses
keyword: two-photon-chromophores
keyword: two-photon-microscopy
landing page: https://theses.hal.science/tel-00863765

Two-photon chromophore-polymer conjugates grafted onto gold nanoparticles as fluorescent probes for bioimaging and photodynamic therapy applications
type: Distribution
format: HTML
issued: 2026-05-09T16:39:01.590331
modified: 2026-05-09T16:39:01.567819
access URL: https://theses.hal.science/tel-00863765

test_moissonnage_selune
type: Agent