Human embryonic pancreas development in a ex vivo / in vivo model
type: Dataset
description: Whilst sporadic human genetic studies have permitted some comparisons between rodent and human pancreatic development, the lack of a robust experimental system has not permitted detailed examination of human pancreatic development. We previously developed a xenograft model of immature human fetal pancreas grafted under the kidney capsule of immune-incompetent mice, which allowed the development of human pancreatic beta cells. Here, we compared the development of human and murine fetal pancreatic grafts either under skeletal muscle epimysium or under the renal capsule. We demonstrated that human pancreatic beta cell development occurs) both by differentiation of pancreatic progenitors and by proliferation of developing beta cells. The superficial location of the skeletal muscle graft and its easier access permitted in vivo lentivirus-mediated gene transfer which targeted specific cells. This model of engraftment under the skeletal muscle epimysium is a new approach for longitudinal studies, which allows localized manipulation to determine the regulation of human pancreatic development.
identifier: NNT: 2013PA05T015
issued: 2026-05-09T17:42:30.318051
language: fr
modified: 2026-05-09T17:42:30.318056
contact point: Organization
  fn: CCSD
keyword: development
keyword: developpement
keyword: infoeu-reposemanticsdoctoralthesis
keyword: pancreas
keyword: sdvmheplife-sciences-q-biohuman-health-and-pathology
keyword: theses
keyword: transgenese
keyword: transgenesis
keyword: xenograft
keyword: xenogreffe
landing page: https://theses.hal.science/tel-00862466

Human embryonic pancreas development in a ex vivo / in vivo model
type: Distribution
format: HTML
issued: 2026-05-09T17:42:30.324211
modified: 2026-05-09T17:42:30.306719
access URL: https://theses.hal.science/tel-00862466

test_moissonnage_selune
type: Agent