EGCG and delphinidin : two new natural inhibitors of hepatitis C virus entry
type: Dataset
description: Hepatitis C is a major global health burden with 160 million infected individuals worldwide. This long-term disease, caused by a small positive-strand ribonucleic acid (RNA) enveloped virus, namely hepatitis C virus (HCV) evolves slowly. Nowadays, no vaccine is available and current treatments are unsatisfactory due to their restricted spectrum of action. For this reason, it is suggested that the combination of several drugs will prevent viral resistance and might conduct to an efficient antiviral therapy. Thus, the discovery of new direct acting antiviral agents (DAAs), with a broad spectrum of action, targeting different steps of the virus life cycle is still needed. Here, we identified (-)-epigallocatechin-3-gallate (EGCG) as a new inhibitor of HCV entry. Epigallocatechin-3-gallate, extracted from green tea, inhibits HCV infection. More precisely, this natural catechin molecule acts at a very early step of entry regardless of the genotype as illustrated with HCV pseudoparticles expressing HCV envelope glycoproteins E1 and E2 assays and cell-cultured HCV assays. Moreover, this molecule inhibits the docking of the virus to the cell surface as showed by the quantification of bound viruses during the attachment step using quantitative real-time polymerase chain reaction. Furthermore, EGCG inhibits viral cell-to-cell transmission as demonstrated by inhibiting cell-free transmission using agarose or neutralizing antibodies assays. Finally, EGCG clears HCV from cell culture supernatants after four passages.The half maximal inhibitory concentration (IC50) of EGCG in cell culture is approximately 11 µM. In order to identify new molecules exhibiting an enhanced anti-HCV activity and displaying similarities from EGCG scaffold, a series of natural compounds were selected and were tested for their anti-HCV activities. Thus, the anthocyanidin delphinidin chloride was identified as another inhibitor of HCV entry. Like EGCG, delphinidin chloride acts directly on the virus at a v…
identifier: NNT: 2012LIL2S031
issued: 2026-05-10T12:11:54.134003
language: fr
modified: 2026-05-10T12:11:54.134007
contact point: Organization
  fn: CCSD
keyword: delphinidine
keyword: egcg
keyword: hepatite-c
keyword: hepatitis-c-virus
keyword: infoeu-reposemanticsdoctoralthesis
keyword: sdvmheplife-sciences-q-biohuman-health-and-pathology
keyword: theses
landing page: https://theses.hal.science/tel-00840465

EGCG and delphinidin : two new natural inhibitors of hepatitis C virus entry
type: Distribution
format: HTML
issued: 2026-05-10T12:11:54.136899
modified: 2026-05-10T12:11:54.120697
access URL: https://theses.hal.science/tel-00840465

test_moissonnage_selune
type: Agent