Silica nanoparticles behaviour in biological medium : from cells to biomaterials
type: Dataset
description: Recent development of nanotechnologies, while promising great advances, in particular in the bio-medical field, rise several questions, e.g. what is the behaviour of these nano-objects in biological conditions? Among these materials, silica arise special interest for it is already used in cosmetics, with the associated question of dermal exposure, and is studied for intracellular drug delivery. This study aims at bringing some answers concerning the fate of different kind of silica nanoparticles in biological conditions by exploring the following phenomena: aggregation and dissolution in biological media, internalisation in 2D or 3D cell cultures, toxicity and intracellular fate. In the context of cutaneous exposure, human dermal cells were used, both in direct exposition and through diffusion of the nanoparticles in dermis-like collagen matrices immobilizing cells. It appears that dissolution is size-dependant, internalisation is influenced by diameter and composition of the particles, toxic effects are governed by number of exposed particles, more than weight concentration, and intracellular dissolution can be modulated by the size of the particles or incorporation of disulfide moieties, possibly inducing the release of encapsulated molecules. Thus characteristics of the nanoparticles (diameter, surface charge and composition) influence their behaviour in biological media and in presence of cells
identifier: NNT: 2012PAO66271
issued: 2026-05-10T15:54:58.139335
language: fr
modified: 2026-05-10T15:54:58.139340
contact point: Organization
  fn: CCSD
keyword: chimmatechemical-sciencesmaterial-chemistry
keyword: diffusion-dans-le-derme
keyword: dissolution-intracellulaire
keyword: infoeu-reposemanticsdoctoralthesis
keyword: internalisation
keyword: nanoparticules-de-silice
keyword: particules-biofragmentables
keyword: theses
keyword: toxicite
landing page: https://theses.hal.science/tel-00836093

Silica nanoparticles behaviour in biological medium : from cells to biomaterials
type: Distribution
format: HTML
issued: 2026-05-10T15:54:58.153237
modified: 2026-05-10T15:54:58.125191
access URL: https://theses.hal.science/tel-00836093

test_moissonnage_selune
type: Agent