Role of caveolin-1 in control of adipocyte lipid storage capacties
type: Dataset
contact point: Organization
  fn: CCSD
keyword: endothelium
keyword: theses
keyword: adipocyte
keyword: sdvmhepphylife-sciences-q-biohuman-health-and-pathologytissues-and-organs-q-bioto
keyword: caveoline-1
keyword: stockage-lipidique
keyword: caveole
keyword: infoeu-reposemanticsdoctoralthesis
keyword: gouttelette-lipidique
landing page: https://theses.hal.science/tel-00827746
description: Caveolin-1 and cavins proteins form membrane caveolae, invaginations of the plasma membrane that are particularly abundant adipocytes and endothelial cells within adipose tissue. Caveolin-1 deficiency leads to lipoatrophy in transgenic mice models suggesting a critical role in the control of lipid storage. Studies on Cav-1 deficient mice model (Cav1 (-/-) mice) also revealed important defects in the cardiovascular function, that are rescued by specific reexpression of Cav1 in endothelium (Cav1 RC mice).To address the contribution of endothelial caveolin-1 expression to the lipoatrophic phenotype, we compared metabolic phenotypes observed in Cav1 deficient with those of Cav1 RC and control mice and found a persistent lipoatrophic phenotype in Cav1 (-/-) mice. Considering the key role of caveolin-1 in the control of adipose tissue lipid storage capacity, we chose an overexpression approach of caveolin-1 and cavins 3T3-L1 adipocytes to investigate the underlying molecular mechanisms. All three cell lines contained a higher number of caveolae whereas only caveolin-1 overexpression led to lipid droplet expansion both in vitro and in vivo, suggesting that caveolin-1 control on lipid storage occurs outside of caveolae. In agreement, larger lipid droplets were found in cells expressing a caveolin-1-perilipin-GFP fusion construct, in which exogenous caveolin-1 strictly localized onto lipid droplets. Together our data demonstrate a specific role of lipid droplet caveolin-1 pool, independent of caveolae to modulate lipid droplet expansibility
identifier: NNT: 2012PA066071
issued: 2026-05-10T23:10:50.028311
language: fr
modified: 2026-05-10T23:10:50.028316

test_moissonnage_selune
type: Agent

Role of caveolin-1 in control of adipocyte lipid storage capacties
type: Distribution
access URL: https://theses.hal.science/tel-00827746
format: HTML
issued: 2026-05-10T23:10:50.041702
modified: 2026-05-10T23:10:50.017398