Assembly and maturation of the bacteriophage T5 capsid : analysis of the expansion and decoration processes
type: Dataset
description: Bacteriophage T5 is a virus infecting E. Coli. Its capsid assembly and maturation include severalcritical steps leading to the formation of new virions during the infectious cycle.During my thesis I focused on the expansion and decoration of T5 capsid. Expansion consists inlarge conformationnal reorganizations of the 775 capsid protein subunits, yielding a two-foldincrease of the capsid volume and allowing it to accomodate the full-length genome. I haveascertained physicochemical conditions that trigger in vitro expansion of the capsid, and using atime-resolved SAXS study, I showed that expansion is a higly cooperative two-state process leadingto a remarkably stable final state. I have also carried out a functional study of the decoration proteinpb10, showing that it only binds to expanded proheads. Finally, a low resolution model of pb10 wasdetermined by SAXS.
identifier: NNT: 2013PA114803
issued: 2026-05-11T06:14:48.208583
language: fr
modified: 2026-05-11T06:14:48.208588
contact point: Organization
  fn: CCSD
keyword: bacteriophage
keyword: capside-virale
keyword: decoration-protein
keyword: expansion
keyword: infoeu-reposemanticsdoctoralthesis
keyword: proteine-de-decoration
keyword: saxs
keyword: sdvsalife-sciences-q-bioagricultural-sciences
keyword: theses
keyword: virus-capsid
landing page: https://theses.hal.science/tel-00819620

Assembly and maturation of the bacteriophage T5 capsid : analysis of the expansion and decoration processes
type: Distribution
format: HTML
issued: 2026-05-11T06:14:48.213842
modified: 2026-05-11T06:14:48.196184
access URL: https://theses.hal.science/tel-00819620

test_moissonnage_selune
type: Agent