Two-photon absorbing fluorophores molecular engineering for biology
type: Dataset
description: Two-photon induced fluorescence is nowadays widely used for the imaging of biological tissues. The classical fluorophores used in confocal microscopy exhibit low sensitivity to two-photon excitation for the two-photon excitation microscopy (TPEM), led the researchers towards the development of new fluorophores, specifically engineered for TPEM. This manuscript describes our work on conception, synthesis and characterizations of new one-dimensional fluorophores based on dicétopyrrolopyrrole (DPP) central core, surrounded by various electro-active systems through π conjugated systems. We also modified such fluorophores to be able to conjugate them to molecules of biological interest, such as proteins. These bio-conjugable fluorophores were grafted on a peptide of HIV virus studied in our laboratory: TAT (Trans-Activator of transcription). The imaging by TPEM was successfully performed on HeLa cells. In addition we developed new multimodal probes for the correlative light electronic microscopy and for the correlative imaging fluorescence microscopy/ Magnetic resonance imaging (MRI). Theses multimodal probes associate a fluorescent moiety based on the DPP core associated to a paramagnetic or electron dense entity (magnetite nanoparticles, gadolinium III or heavy atoms such as platinum or gold).
identifier: NNT: 2012STRAJ051
issued: 2026-05-11T15:17:05.906832
language: fr
modified: 2026-05-11T15:17:05.906837
contact point: Organization
  fn: CCSD
keyword: absorption-a-deux-photons
keyword: bio-conjugable-fluorophores
keyword: chimorgachemical-sciencesorganic-chemistry
keyword: dicetopyrrelopyrrole
keyword: diketopyrrolopyrrole
keyword: fluorescent-dyes
keyword: fluorophores-bio-conjugables
keyword: infoeu-reposemanticsdoctoralthesis
keyword: ingenierie-moleculaire
keyword: microscopie-a-deux-photons
keyword: molecular-engineering
keyword: non-linear-optic
keyword: optique-non-lineaire
keyword: sdvaenlife-sciences-q-biofood-and-nutrition
keyword: sonde-fluorescente
keyword: theses
keyword: two-photon-absorption
keyword: two-photon-microscopy
landing page: https://theses.hal.science/tel-00809524

Two-photon absorbing fluorophores molecular engineering for biology
type: Distribution
format: HTML
issued: 2026-05-11T15:17:05.914789
modified: 2026-05-11T15:17:05.885330
access URL: https://theses.hal.science/tel-00809524

test_moissonnage_selune
type: Agent