Structural and functional studies of MexAB-OprM efflux pump involved in Pseudomonas aeruginosa antibiotics resistance
type: Dataset
description: Pseudomonas aeruginosa is an opportunistic pathogen involved in nosocomial infections. This bacteria has developed various strategies to resist antibiotics treatments, one of them being the activation of membrane efflux pumps. These tripartite systems consist of an OMF (Outer Membrane Factor) family porin, localized in the outer membrane, an active transporter in the inner membrane, belonging to the RND (Resistance Nodulation Division) family and a periplasmic adaptator protein, member of the MFP (Membrane Fusion Protein) family which consolidates the whole complex. Results obtained during this thesis contribute to a better understanding of efflux pumps’ assembly and opening thanks to the development of new research tools borrowed from physic, biochemistry and microbiology. The first study describes the binding stoechiometry of MexA with its cognate partner OprM by Blue Native Polyacrylamide gel Electrophoresis (Ferrandez, Monlezun et al. 2012). Secondly, a study, in collaboration with B. Le Pioufle’s team (ENS Cachan), was dedicated to the electrophysiologic caracterization of OprM opening using a microfluidic device incorporated with a miniaturized artificial bilayer membrane (Wang, Monlezun et al. 2012). Then, to complete this analysis in vivo, in the third part of this thesis, complementation experiments were initiated in a Pseudomonas aeruginosa strain deleted of its chromosomal oprM gene. Finally, in collaboration with P. Plésiat’s team (Laboratoire de Bactériologie, Besançon), modelling of MexZ, the MexXY/OprM pump’s regulator and modelling of the carbapenems’ porin OprD were made in order to link structural modifications to mutations observed in clinical strains.
identifier: NNT: 2012PA05P644
issued: 2026-05-12T10:48:45.831050
language: fr
modified: 2026-05-12T10:48:45.831057
contact point: Organization
  fn: CCSD
keyword: antibiotics-resistance
keyword: bn-page
keyword: bnpage
keyword: complementation-in-vivo
keyword: cristallisation
keyword: crystallisation
keyword: efflux-pump
keyword: electrophysiologie
keyword: electrophysiology
keyword: in-vivo-complementation
keyword: infoeu-reposemanticsdoctoralthesis
keyword: membrane-proteins
keyword: modelisation
keyword: modelling
keyword: pompe-defflux
keyword: proteines-membranaires
keyword: resistance-aux-antibiotiques
keyword: sdvmheplife-sciences-q-biohuman-health-and-pathology
keyword: theses
landing page: https://theses.hal.science/tel-00801703

Structural and functional studies of MexAB-OprM efflux pump involved in Pseudomonas aeruginosa antibiotics resistance
type: Distribution
format: HTML
issued: 2026-05-12T10:48:45.850038
modified: 2026-05-12T10:48:45.779635
access URL: https://theses.hal.science/tel-00801703

test_moissonnage_selune
type: Agent