Development of novel analytical tools based on nucleic acid aptamers for the detection of small molecules
type: Dataset
description: Small biomolecule detection is of great interest and importance in the pharmaceutical, environmental, food and clinical fields. Aptamers, selected by SELEX (Systematic Evolution of Ligands by Exponential Enrichment), are oligonucleotides that bind to a target with high affinity and specificity. The objective of the work is to establish novel methodologies of aptamer-based assays for the small biomolecule detection. In the first work, a rationalized capillary electrophoresis strategy, derived from the structure-switching aptamer concept, is described for the design of simultaneous detection of multiple analytes. The second work based on a gold nanoparticle colorimetric sensing strategy allows a rapid, label-free, homogeneous assay for small molecule using an aptamer enzymatic cleavage protection strategy. In the third work, two aptamer-based fluorescence polarization approaches, using the displacement concept, are described to improve the potentialities of the small molecule-dedicated aptasensors.
identifier: NNT: 2012GRENV032
issued: 2026-05-15T13:55:16.091533
language: fr
modified: 2026-05-15T13:55:16.091538
contact point: Organization
  fn: CCSD
keyword: acide-nucleique-aptameres
keyword: capillary-electrophoresis
keyword: electrophorese-capillaire
keyword: fluorescence-polarization
keyword: gold-nanoparticles
keyword: infoeu-reposemanticsdoctoralthesis
keyword: nanoparticules-dor
keyword: nucleic-acid-aptamers
keyword: petites-molecules
keyword: polarisation-de-fluorescence
keyword: sdvsalife-sciences-q-bioagricultural-sciences
keyword: selex
keyword: small-molecules
keyword: theses
landing page: https://theses.hal.science/tel-00770171

Development of novel analytical tools based on nucleic acid aptamers for the detection of small molecules
type: Distribution
format: HTML
issued: 2026-05-15T13:55:16.101745
modified: 2026-05-15T13:55:16.061798
access URL: https://theses.hal.science/tel-00770171

test_moissonnage_selune
type: Agent