Structure and function of a ESCRT-III ligand, LgD/CC2D1A, involved in HIV virus budding
type: Dataset
description: Budding is the final step of HIV infection. Viral particles will have to modify the topology of the plasma membrane in order to achieve their correct release from the infected cell, by recruiting ESCRT proteins at the budding point, and among them CHMP4 and CHMP2 isoforms. So far, the molecular details of this recruitment are not precisely known.. Lethal Giant Discs (LgD) has been descibed in the litterature as a regulator of endosomal trafficking, and an interaction with CHMP4B has been proposed. A major point of this research is to propose a structural basis for this interaction, as well as a better understanding of the role and general organization of LgD/CC2D1A. The crystal structure of a LgD fragment (comprising a predicted coiled-coil motif and a c-terminal C2 domain) was solved in our lab at 2.4 A. Moreover, we show that CC2D1A impairs in vitro the ability of CHMP4B to polymerize. Based on a crystallographic structure of CHMP4B and biochemical data, we also show that the binding site of CC2D1A on CHMP4B is itself involved in polymerization, in the context of HIV budding. As a side project, I've also set up a protocole to obtain pure monomeric CHMP2B, which has been shown to polymerize at the plasma membrane, and I've characterized the protein in the presence of liposomes, along with new partners.
identifier: NNT: 2011GRENV078
issued: 2026-05-29T03:03:38.535378
language: fr
modified: 2026-05-29T03:03:38.535384
contact point: Organization
  fn: CCSD
keyword: bourgeonnement
keyword: budding
keyword: complexe-de-proteines
keyword: cristallographie
keyword: endosomal-pathway
keyword: escrt
keyword: hiv
keyword: infoeu-reposemanticsdoctoralthesis
keyword: protein-complex
keyword: sdvbbmbslife-sciences-q-biobiochemistry-molecular-biologystructural-biology-q-biobm
keyword: theses
keyword: vih
keyword: voie-endosomale
landing page: https://theses.hal.science/tel-00769410

Structure and function of a ESCRT-III ligand, LgD/CC2D1A, involved in HIV virus budding
type: Distribution
format: HTML
issued: 2026-05-29T03:03:38.592913
modified: 2026-05-29T03:03:38.491289
access URL: https://theses.hal.science/tel-00769410

test_moissonnage_selune
type: Agent