The synthesis of hybrid biomimetic nanostructures (calcium phosphates + proteins) by advanced laser techniques : structural, biochemical and biological characterization
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access URL: https://theses.hal.science/tel-00703266
format: HTML
issued: 2026-05-16T09:21:36.479216
modified: 2026-05-16T09:21:36.387380

test_moissonnage_selune
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The synthesis of hybrid biomimetic nanostructures (calcium phosphates + proteins) by advanced laser techniques : structural, biochemical and biological characterization
type: Dataset
contact point: Organization
  fn: CCSD
keyword: infoeu-reposemanticsdoctoralthesis
keyword: vitronectine
keyword: cellular-proliferation-and-differentiation
keyword: proliferation-et-differentiation-cellulaires
keyword: thin-films
keyword: biocompatibilite
keyword: fibronectin
keyword: nanostructures-hybrides-biomimetiques
keyword: couches-minces
keyword: biocompatibility
keyword: fibronectine
keyword: osseous-implant
keyword: pulsed-lasers
keyword: lasers-pulses
keyword: hydroxyapatite
keyword: biomimetic-hybrid-nanostructures
keyword: physcondcm-genphysics-physicscondensed-matter-cond-matother-cond-matother
keyword: theses
keyword: implant-osseux
keyword: vitronectin
landing page: https://theses.hal.science/tel-00703266
description: The work presented within the thesis concern the fabrication of biomimetic nanostructured biomaterial thin films by pulsed laser techniques and their evaluation from the physico-chemical and biological (cellular biocompatibility, proliferation and differentiation) points of view. The aim is to develop a new method for coating the osseous implants by advanced pulsed laser techniques (PLD – pulsed laser deposition and MAPLE – matrix assisted pulsed laser evaporation). These techniques are used for the fabrication of a biphasic system composed of hydroxyapatite (HA) nanoparticules associated with large adhesion proteins as e.g. fibronectin (FN) and vitronectin (VN) deposited on a titanium substrate. The metallic substrate will allow keeping the mechanical rigidity; the hydroxyapatite will favor the bio-integration in the osseous tissue while the proteins will accelerate the cellular adhesion. The main objective is to speed up the cellular adhesion and the formation of new tissue around the implant. The cellular proliferation and differentiation studies demonstrated a predisposal to cell proliferation induced by the VN coatings and to cell differentiation by FN. The significant effects on the cell adhesion, proliferation and differentiation observed in our studies are of great importance for the mechanical stability phase of the implant. The layers HA/proteins deposited by laser could reduce the time of this phase.
identifier: NNT: 2011MULH5431
issued: 2026-05-16T09:21:36.429235
language: fr
modified: 2026-05-16T09:21:36.429241