test_moissonnage_selune
type: Agent

oai-hal-tel-00689788v1
type: Dataset
contact point: Organization
  fn: CCSD
keyword: ble
keyword: plantes
keyword: sdvbvlife-sciences-q-biovegetal-biology
keyword: graminees
keyword: genetique-moleculaire-vegetale
keyword: ressources-genetiques
keyword: riz
keyword: marqueurs-genetiques
keyword: theses
keyword: amelioration-genetique
keyword: infoeu-reposemanticsdoctoralthesis
landing page: https://theses.hal.science/tel-00689788
description: Despite recent progress in wheat (T. aestivum L.) genomics, only few efforts have been made in the genomics of wild and closely related wheat species. The first aim of this PhD was to develop new molecular markers useful on wheat and transferable to other cultivated as well as wild grass species. We focused our work on microsatellites (SSRs) located in expressed gene sequences (ESTs) to exploit the large number of wheat ESTs present in the databases and also because the genes are the best conserved regions of the genome between the different species. From about 1,000 EST contigs bearing a microsatellite, we observed that the trinucleotide motifs were the most frequent (~80%). We developed 301 primer pairs (EST-SSRs) that were evaluated on a set of eight wheat cultivars and eight related species. About 80% of the primer pairs gave an amplification product on wheat. Most of the time, we had high quality patterns. The level of polymorphism was estimated to be 25.4% on wheat (mean of 3.1 allele/locus and PIC value of 0.40). Among the 240 EST-SSRs that gave an amplification product on wheat, 177 were assigned to wheat chromosomes using aneuploid lines and 81 new loci were added and integrated to the reference wheat genetic maps. Transferability of wheat EST-SSRs to related species was very good for wheat subspecies (100% for T. aestivum ssp compactum) and decreased while the phylogenetic distance increased. However, transferability reached 28% with rice. The alignment of the selected wheat EST-SSRs on the rice genome sequence showed a preferential localisation in the telomeric regions and a better similarity of wheat ESTs on rice chromosomes 2, 3 and 5. Moreover, a thorough study of the EST-SSRs giving an amplification product in wheat, rice and barley indicates that the motif is present and is frequently similar in the three species suggesting a common ancestral origin. The allelic diversity of the EST-SSRs among the wheat related species was higher in allogamous specie…
identifier: NNT: 2006CLF21650
issued: 2026-05-21T06:14:10.002978
language: en
modified: 2026-05-21T06:14:10.002984
title: Study of the transferability of microsatellite markers derived from bread wheat (T. aestivum) or rice ( O. sativa) ESTs (EST-SSRs) to their close and wild relative and evaluation of their potential for the organisation of genetic resources in the grass family

https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-tel-00689788v1/resource/a7aab967-b3d9-4227-9fb0-01220d895fde
type: Distribution
access URL: https://theses.hal.science/tel-00689788
format: HTML
issued: 2026-05-21T06:14:10.011458
modified: 2026-05-21T06:14:09.969435
title: Study of the transferability of microsatellite markers derived from bread wheat (T. aestivum) or rice ( O. sativa) ESTs (EST-SSRs) to their close and wild relative and evaluation of their potential for the organisation of genetic resources in the grass family