This work established the identity card of nanoparticles developped in our laboratory; these nanoparticles are called lipidic nanocapsules. Indeed, these lipidic nanocapsules were characterized by dynamic light scattering and also by small angle neutron scattering. These experiments have demonstrated the structure of the lipidic nanocapsules as core-shell systems: an oily core surrounded by a mix monolayer of surfactants. By the way, starting from these core-shell systems, we have developped two methodologies able to produce multifunctional lipidic nanocapusles. The first one is the post-insertion process, initialy developped onto liposomes; the second one is a new process based on a transacylation reaction between the surfactants of lipidic nanocapsules and an another molecule presenting a high interest towards pharmaceuticals sciences.We have demonstrated that these new nanocapsules had new properties such the ability to deliver small interfering RNA (siRNA) into mammalian cells.
Modifications of lipidic nanocapsules by post-formulation processes. Creation of multifunctional nanoparticles evaluated as drug vectors.