Bacteriophage SPP1 entry into the host cell
type: Dataset
description: The four main steps of bacterial viruses (bacteriophages) lytic infection are (i) specific recognition and genome entry into the host bacterium, (ii) replication of the viral genome, (iii) assembly of viral particles, and (iv) their release, leading in most cases to cell lysis. Although the course of individual steps of the viral infection cycle has been relatively well established, the details of how viral DNA transits from the virion to the host cytoplasm and of how the cellular environment catalyzes and possibly organizes the entire process remain poorly understood.Tailed bacteriophages are by far the most abundant viruses that infect Eubacteria. The first event in their infection is recognition of a receptor on the surface of host bacterium by the phage adsorption machinery. The barriers that the infectious particle overcomes subsequently are the cell wall and the cytoplasmic membrane of bacteria. This implies a localized degradation of the wall and the flow of its double stranded DNA (dsDNA) through a hydrophilic pore in the membrane. The lineards DNA molecule is most frequently circularized in the cytoplasm followed by its replication. In this study we used bacteriophage SPP1 that infects the Gram-positive bacterium Bacillus subtilis as a model system to dissect the different steps leading to transfer of the phage genome from the viral capsid to the host cell cytoplasm.normally to B. subtilis but do not trigger depolarization of the CM. Attachment of intact SPP1 particles is thus required for phage-induced depolarization.The beginning of B. subtilis infection by bacteriophage SPP1 was followed inspace and time. The position of SPP1 binding at the cell surface was imaged by fluorescence microscopy using virus particles labeled with "quantum dots". We found that SPP1 reversible adsorption occurs preferentially at the cell poles. This initial binding facilitates irreversible adsorption to the SPP1 phage receptor protein YueB,which is encoded by a putative type VI…
landing page: https://theses.hal.science/tel-00669654
identifier: NNT: 2011PA114842
keyword: bacteriophage-spp1
keyword: ca2-ions
keyword: entree-du-virus
keyword: gram-positive
keyword: infoeu-reposemanticsdoctoralthesis
keyword: ions-calcium
keyword: membrane-voltage
keyword: sdvsalife-sciences-q-bioagricultural-sciences
keyword: theses
keyword: virus-entry
keyword: yueb
issued: 2026-05-28T21:24:01.912986
modified: 2026-05-28T21:24:01.912991
language: en
contact point: Organization
  fn: CCSD

test_moissonnage_selune
type: Agent

Bacteriophage SPP1 entry into the host cell
type: Distribution
format: HTML
access URL: https://theses.hal.science/tel-00669654
issued: 2026-05-28T21:24:02.125531
modified: 2026-05-28T21:24:01.862135