@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-tel-00092909v1> a dcat:Dataset ;
    dct:description """
              Nowadays biology research is faced with a considerable amount of genomic data which has to be functionally characterised in order to bring new concepts, which will lead to new therapies. These studies approach molecular-based mechanisms, and need high-throughput and parallel data management. The multidisciplinary work presented here has allowed the development of a miniaturised technology of cell culture in liquid drops, matrixed on a plane solid substrate, to test the action of molecules or conditions on the cells behaviour. <br />After the macroscopic demonstration of nucleic acid molecule transfection in living cells on such a device, we have developed a fabrication protocol for a miniaturised support, able to maintain 100 nano-drops per cm², based on differential surface tensions. A picoliter dispensing robot was integrated to make the cell culture drops automatically. The cells' behaviour in the drops is analysed by high-content fluorescence microscopy after fixation. Each cell of each drop is characterised by tenths of parameters individually. <br />This new analytical approach, which has triggered the development of new bio-statistical tools, has been applied to a multipartner project of siRNA screening, aimed at studying the impact of genes on glioblastoma cells chemoresistance. We have also shown the use of mass spectrometry as a multiparametric phenotyping method. This cell-on-chip technology seems particularly well suited for the study of the precise behaviour of cells among a population at high-throughput. It will be the base of new lab-on-chip technologies.
            """ ;
    dct:identifier "tel-00092909" ;
    dct:issued "2026-05-07T11:41:13.132104"^^xsd:dateTime ;
    dct:language "fr" ;
    dct:modified "2026-05-07T11:41:13.132108"^^xsd:dateTime ;
    dct:publisher <https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> ;
    dct:title "Development of a new cell-on-chip format for high-content phenotyping" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "CCSD" ] ;
    dcat:distribution <https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-tel-00092909v1/resource/18b45b78-5d61-4cbc-b240-fe9dcc1177f4> ;
    dcat:keyword "biologie-cellulaire",
        "cell-biology",
        "cell-on-chip",
        "criblage-haut-debit",
        "haut-contenu",
        "high-content",
        "high-throughput-screening",
        "infoeu-reposemanticsdoctoralthesis",
        "mass-spectrometry",
        "nanotechnologie",
        "nanotechnology",
        "puce-a-cellules",
        "rna-interference",
        "sdvlife-sciences-q-bio",
        "spectrometrie-de-masse",
        "surface-tension",
        "tensions-de-surface",
        "theses" ;
    dcat:landingPage <https://theses.hal.science/tel-00092909> .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-tel-00092909v1/resource/18b45b78-5d61-4cbc-b240-fe9dcc1177f4> a dcat:Distribution ;
    dct:format "HTML" ;
    dct:issued "2026-05-07T11:41:13.144719"^^xsd:dateTime ;
    dct:modified "2026-05-07T11:41:13.113172"^^xsd:dateTime ;
    dct:title "Development of a new cell-on-chip format for high-content phenotyping" ;
    dcat:accessURL <https://theses.hal.science/tel-00092909> .

<https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> a foaf:Agent ;
    foaf:name "test_moissonnage_selune" .

<https://theses.hal.science/tel-00092909> a foaf:Document .