@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-pasteur-00976043v1> a dcat:Dataset ;
    dct:description """
              Unlike superoxide dismutases (SODs), superoxide reductases (SORs) eliminate superoxide anion (O(2)(*-)) not through its dismutation, but via reduction to hydrogen peroxide (H(2)O(2)) in the presence of an electron donor. The microaerobic protist Giardia intestinalis, responsible for a common intestinal disease in humans, though lacking SOD and other canonical reactive oxygen species-detoxifying systems, is among the very few eukaryotes encoding a SOR yet identified. In this study, the recombinant SOR from Giardia (SOR(Gi)) was purified and characterized by pulse radiolysis and stopped-flow spectrophotometry. The protein, isolated in the reduced state, after oxidation by superoxide or hexachloroiridate(IV), yields a resting species (T(final)) with Fe(3+) ligated to glutamate or hydroxide depending on pH (apparent pK(a)=8.7). Although showing negligible SOD activity, reduced SOR(Gi) reacts with O(2)(*-) with a pH-independent second-order rate constant k(1)=1.0×10(9) M(-1) s(-1) and yields the ferric-(hydro)peroxo intermediate T(1); this in turn rapidly decays to the T(final) state with pH-dependent rates, without populating other detectable intermediates. Immunoblotting assays show that SOR(Gi) is expressed in the disease-causing trophozoite of Giardia. We propose that the superoxide-scavenging activity of SOR in Giardia may promote the survival of this air-sensitive parasite in the fairly aerobic proximal human small intestine during infection.
            """ ;
    dct:identifier "pasteur-00976043" ;
    dct:issued "2026-05-05T15:46:49.348079"^^xsd:dateTime ;
    dct:language "en" ;
    dct:modified "2026-05-05T15:46:49.348084"^^xsd:dateTime ;
    dct:publisher <https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> ;
    dct:title "The superoxide reductase from the early diverging eukaryote Giardia intestinalis." ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "CCSD" ] ;
    dcat:distribution <https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-pasteur-00976043v1/resource/65801628-ee2e-4ca9-8756-08a99419d1a6> ;
    dcat:keyword "infoeu-reposemanticsarticle",
        "journal-articles",
        "mesh-cells-cultured",
        "mesh-cloning-molecular",
        "mesh-eukaryota",
        "mesh-giardia-lamblia",
        "mesh-giardiasis",
        "mesh-humans",
        "mesh-hydrogen-ion-concentration",
        "mesh-hydrogen-peroxide",
        "mesh-intestine-small",
        "mesh-iridium",
        "mesh-iron",
        "mesh-oxidoreductases",
        "mesh-phylogeny",
        "mesh-protozoan-proteins",
        "mesh-pulse-radiolysis",
        "mesh-recombinant-proteins",
        "mesh-spectrophotometry",
        "mesh-superoxide-dismutase",
        "sdvbbmlife-sciences-q-biobiochemistry-molecular-biology" ;
    dcat:landingPage <ISSN:%200891-5849> .

<ISSN:%200891-5849> a foaf:Document .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-pasteur-00976043v1/resource/65801628-ee2e-4ca9-8756-08a99419d1a6> a dcat:Distribution ;
    dct:format "HTML" ;
    dct:issued "2026-05-05T15:46:49.360492"^^xsd:dateTime ;
    dct:modified "2026-05-05T15:46:49.327749"^^xsd:dateTime ;
    dct:title "The superoxide reductase from the early diverging eukaryote Giardia intestinalis." ;
    dcat:accessURL <https://riip.hal.science/pasteur-00976043> .

<https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> a foaf:Agent ;
    foaf:name "test_moissonnage_selune" .