@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-pasteur-00975905v1> a dcat:Dataset ;
    dct:description """
              The icsA gene of Shigella encodes a structural protein involved in colonization of the intestinal mucosa by bacteria. This gene is expressed upon invasion of the host and is controlled by a complex regulatory circuit involving the nucleoid protein H-NS, the AraC-like transcriptional activator VirF, and a 450 nt antisense RNA (RnaG) acting as transcriptional attenuator. We investigated on the interplay of these factors at the molecular level. DNase I footprints reveal that both H-NS and VirF bind to a region including the icsA and RnaG promoters. H-NS is shown to repress icsA transcription at 30°C but not at 37°C, suggesting a significant involvement of this protein in the temperature-regulated expression of icsA. We also demonstrate that VirF directly stimulates icsA transcription and is able to alleviate H-NS repression in vitro. According to these results, icsA expression is derepressed in hns- background and overexpressed when VirF is provided in trans. Moreover, we find that RnaG-mediated transcription attenuation depends on 80 nt at its 5'-end, a stretch carrying the antisense region. Bases engaged in the initial contact leading to sense-antisense pairing have been identified using synthetic RNA and DNA oligonucleotides designed to rebuild and mutagenize the two stem-loop motifs of the antisense region.
            """ ;
    dct:identifier "pasteur-00975905" ;
    dct:issued "2026-05-05T15:48:25.224333"^^xsd:dateTime ;
    dct:language "en" ;
    dct:modified "2026-05-05T15:48:25.224338"^^xsd:dateTime ;
    dct:publisher <https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> ;
    dct:title "A multifactor regulatory circuit involving H-NS, VirF and an antisense RNA modulates transcription of the virulence gene icsA of Shigella flexneri." ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "CCSD" ] ;
    dcat:distribution <https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-pasteur-00975905v1/resource/750492ca-dffb-4077-904a-8618423df134> ;
    dcat:keyword "infoeu-reposemanticsarticle",
        "journal-articles",
        "mesh-bacterial-proteins",
        "mesh-base-sequence",
        "mesh-binding-sites",
        "mesh-dna-binding-proteins",
        "mesh-gene-expression-regulation-bacterial",
        "mesh-molecular-sequence-data",
        "mesh-promoter-regions-genetic",
        "mesh-rna-antisense",
        "mesh-rna-bacterial",
        "mesh-trans-activators",
        "mesh-transcription-factors",
        "mesh-transcription-genetic",
        "mesh-virulence-factors",
        "sdvmplife-sciences-q-biomicrobiology-and-parasitology" ;
    dcat:landingPage <ISSN:%200305-1048> .

<ISSN:%200305-1048> a foaf:Document .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-pasteur-00975905v1/resource/750492ca-dffb-4077-904a-8618423df134> a dcat:Distribution ;
    dct:format "HTML" ;
    dct:issued "2026-05-05T15:48:25.233547"^^xsd:dateTime ;
    dct:modified "2026-05-05T15:48:25.210613"^^xsd:dateTime ;
    dct:title "A multifactor regulatory circuit involving H-NS, VirF and an antisense RNA modulates transcription of the virulence gene icsA of Shigella flexneri." ;
    dcat:accessURL <https://riip.hal.science/pasteur-00975905> .

<https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> a foaf:Agent ;
    foaf:name "test_moissonnage_selune" .