@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

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    dct:description """
              The biphenyl dioxygenase of Burkholderia xenovorans LB400 (BphAE(LB400)) is a Rieske-type oxygenase that catalyzes the stereospecific oxygenation of many heterocyclic aromatics including dibenzofuran. In a previous work, we evolved BphAE(LB400) and obtained BphAE(RR41). This variant metabolizes dibenzofuran and 2-chlorodibenzofuran more efficiently than BphAE(LB400). However, the regiospecificity of BphAE(RR41) toward these substrates differs. Dibenzofuran is metabolized principally through a lateral dioxygenation whereas 2-chlorodibenzofuran is metabolized principally through an angular dioxygenation. In order to explain this difference, we examined the crystal structures of both substrate-bound forms of BphAE(RR41) obtained under anaerobic conditions. This structure analysis, in combination with biochemical data for a Ser283Gly mutant provided evidences that the substrate is compelled to move after oxygen-binding in BphAE(RR41):dibenzofuran. In BphAE(RR41):2-chlorodibenzofuran, the chlorine atom is close to the side chain of Ser283. This contact is missing in the BphAE(RR41):dibenzofuran, and strong enough in the BphAE(RR41):2-chlorodibenzofuran to help prevent substrate movement during the catalytic reaction.
            """ ;
    dct:identifier "pasteur-00818384" ;
    dct:issued "2026-05-11T07:23:02.582483"^^xsd:dateTime ;
    dct:language "en" ;
    dct:modified "2026-05-11T07:23:02.582488"^^xsd:dateTime ;
    dct:publisher <https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> ;
    dct:title "Structural insights into the metabolism of 2-chlorodibenzofuran by an evolved biphenyl dioxygenase." ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "CCSD" ] ;
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    dcat:keyword "infoeu-reposemanticsarticle",
        "infoinfo-btcomputer-science-csbiotechnology",
        "journal-articles",
        "mesh-benzofurans",
        "mesh-burkholderia",
        "mesh-catalysis",
        "mesh-crystallization",
        "mesh-dioxygenases",
        "mesh-glycine",
        "mesh-mutation",
        "mesh-protein-conformation",
        "mesh-serine",
        "sdvbiolife-sciences-q-biobiotechnology" ;
    dcat:landingPage <ISSN:%200006-291X> .

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<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-pasteur-00818384v1/resource/0f849303-a9c2-4fe7-8be9-680c707810e5> a dcat:Distribution ;
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    dct:issued "2026-05-11T07:23:02.599052"^^xsd:dateTime ;
    dct:modified "2026-05-11T07:23:02.569310"^^xsd:dateTime ;
    dct:title "Structural insights into the metabolism of 2-chlorodibenzofuran by an evolved biphenyl dioxygenase." ;
    dcat:accessURL <https://riip.hal.science/pasteur-00818384> .

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