Structural insights into the metabolism of 2-chlorodibenzofuran by an evolved biphenyl dioxygenase.
type: Dataset
description: The biphenyl dioxygenase of Burkholderia xenovorans LB400 (BphAE(LB400)) is a Rieske-type oxygenase that catalyzes the stereospecific oxygenation of many heterocyclic aromatics including dibenzofuran. In a previous work, we evolved BphAE(LB400) and obtained BphAE(RR41). This variant metabolizes dibenzofuran and 2-chlorodibenzofuran more efficiently than BphAE(LB400). However, the regiospecificity of BphAE(RR41) toward these substrates differs. Dibenzofuran is metabolized principally through a lateral dioxygenation whereas 2-chlorodibenzofuran is metabolized principally through an angular dioxygenation. In order to explain this difference, we examined the crystal structures of both substrate-bound forms of BphAE(RR41) obtained under anaerobic conditions. This structure analysis, in combination with biochemical data for a Ser283Gly mutant provided evidences that the substrate is compelled to move after oxygen-binding in BphAE(RR41):dibenzofuran. In BphAE(RR41):2-chlorodibenzofuran, the chlorine atom is close to the side chain of Ser283. This contact is missing in the BphAE(RR41):dibenzofuran, and strong enough in the BphAE(RR41):2-chlorodibenzofuran to help prevent substrate movement during the catalytic reaction.
issued: 2026-05-11T07:23:02.582483
language: en
modified: 2026-05-11T07:23:02.582488
contact point: Organization
  fn: CCSD
keyword: infoeu-reposemanticsarticle
keyword: infoinfo-btcomputer-science-csbiotechnology
keyword: journal-articles
keyword: mesh-benzofurans
keyword: mesh-burkholderia
keyword: mesh-catalysis
keyword: mesh-crystallization
keyword: mesh-dioxygenases
keyword: mesh-glycine
keyword: mesh-mutation
keyword: mesh-protein-conformation
keyword: mesh-serine
keyword: sdvbiolife-sciences-q-biobiotechnology
landing page: 0006-291X

Structural insights into the metabolism of 2-chlorodibenzofuran by an evolved biphenyl dioxygenase.
type: Distribution
format: HTML
issued: 2026-05-11T07:23:02.599052
modified: 2026-05-11T07:23:02.569310
access URL: https://riip.hal.science/pasteur-00818384

test_moissonnage_selune
type: Agent