Two-Photon Imaging
type: Distribution
access URL: https://inserm.hal.science/inserm-00771945
format: HTML
issued: 2026-05-15T11:22:16.031176
modified: 2026-05-15T11:22:15.986703

Two-Photon Imaging
type: Dataset
contact point: Organization
  fn: CCSD
keyword: sdvneulife-sciences-q-bioneurons-and-cognition-q-bionc
keyword: neurons
keyword: neuroimaging
keyword: infoeu-reposemanticsbookpart
keyword: intravital-imaging
keyword: second-harmonic-generation
keyword: neuro-oncology
keyword: glial-cells
keyword: nonlinear-optics
keyword: microscopy
keyword: fluorescence
keyword: neuropathologies
keyword: book-sections
keyword: instrumentation
keyword: microvasculature
landing page: Encyclopedia of Neuroscience
description: During the past two decades, two-photon microscopy has become a powerful tool in neuroscience. Unlike other imaging methods like MRI, its spatial resolution is micrometric and enables the observation of structures at the subcellular scale. In this chapter, the physical principles and the way to study phenomenon occurring in the living animal are summarized. Then, we describe the methods to observe the different components of the nervous system like neurons, glia and brain microvasculature. Finally, we discuss the advantages of two-photon microscopy in neuro-oncology and in the study of diseases.
issued: 2026-05-15T11:22:16.022298
language: en
modified: 2026-05-15T11:22:16.022304

test_moissonnage_selune
type: Agent