Dicistronic MLV-retroviral vectors transduce neural precursors in vivo and co-express two genes in their differentiated neuronal progeny.
type: Dataset
contact point: Organization
  fn: CCSD
keyword: mesh-kanamycin-kinase
keyword: mesh-neurons
keyword: mesh-cerebellum
keyword: mesh-animals
keyword: mesh-stem-cells
keyword: mesh-moloney-murine-leukemia-virus
keyword: mesh-isoenzymes
keyword: sdvmhepmilife-sciences-q-biohuman-health-and-pathologyinfectious-diseases
keyword: infoeu-reposemanticsarticle
keyword: sdvneulife-sciences-q-bioneurons-and-cognition-q-bionc
keyword: mesh-genetic-vectors
keyword: mesh-nih-3t3-cells
keyword: mesh-research-support-non-us-govt
keyword: mesh-mice
keyword: mesh-cell-differentiation
keyword: journal-articles
keyword: sdvgenlife-sciences-q-biogenetics
keyword: mesh-transgenes
keyword: mesh-transduction-genetic
landing page: 1742-4690
description: Dicistronic MLV-based retroviral vectors, in which two IRESes independently initiate the translation of two proteins from a single RNA, have been shown to direct co-expression of proteins in several cell culture systems. Here we report that these dicistronic retroviral vectors can drive co-expression of two gene products in brain cells in vivo. Injection of retroviral vector producer cells leads to the transduction of proliferating precursors in the external granular layer of the cerebellum and throughout the ventricular regions. Differentiated neurons co-expressing both transgenes were observed in the cerebellum and in lower numbers in distant brain regions such as the cortex. Thus, we describe an eukaryotic dicistronic vector system that is capable of transducing mouse neural precursors in vivo and maintaining the expression of genes after cell differentiation.
issued: 2026-05-08T10:29:46.988670
language: en
modified: 2026-05-08T10:29:46.988675

Dicistronic MLV-retroviral vectors transduce neural precursors in vivo and co-express two genes in their differentiated neuronal progeny.
type: Distribution
access URL: https://inserm.hal.science/inserm-00089922
format: HTML
issued: 2026-05-08T10:29:47.021756
modified: 2026-05-08T10:29:46.964184

test_moissonnage_selune
type: Agent