Optical imaging of luminescence for in vivo quantification of gene electrotransfer in mouse muscle and knee.
type: Dataset
description: BACKGROUND: Optical imaging is an attractive non-invasive way to evaluate the expression of a transferred DNA, mainly thanks to its lower cost and ease of realization. In this study optical imaging was evaluated for monitoring and quantification of the mouse knee joint and tibial cranial muscle electrotransfer of a luciferase encoding plasmid. Optical imaging was applied to study the kinetics of luciferase expression in both tissues. RESULTS: The substrate of luciferase (luciferin) was injected either intraperitonealy (i.p.) or in situ into the muscle or the knee joint. Luminescence resulting from the luciferase-luciferin reaction was measured in vivo with a cooled CCD camera and/or in vitro on tissue lysate. Maximal luminescence of the knee joint and muscle after i.p. (2.5 mg) or local injection of luciferin (50 microg in the knee joint, 100 microg in the muscle) were highly correlated. With the local injection procedure adopted, in vivo and in vitro luminescences measured on the same muscles significantly correlated. Luminescence measurements were reproducible and the signal level was proportional to the amount of plasmid injected. In vivo luciferase activity in the electrotransfered knee joint was detected for two weeks. Intramuscular electrotransfer of 0.3 or 3 microg of plasmid led to stable luciferase expression for 62 days, whereas injecting 30 microg of plasmid resulted in a drop of luminescence three weeks after electrotransfer. These decreases were partially associated with the development of an immune response. CONCLUSION: A particular advantage of the i.p. injection of substrate is a widespread distribution at luciferase production sites. We have also highlighted advantages of local injection as a more sensitive detection method with reduced substrate consumption. Besides, this route of injection is relatively free of uncontrolled parameters, such as diffusion to the target organ, crossing of biological barriers and evidencing variations in local enzymat…
landing page: 1472-6750
keyword: infoeu-reposemanticsarticle
keyword: infoinfo-btcomputer-science-csbiotechnology
keyword: journal-articles
keyword: mesh-animals
keyword: mesh-comparative-study
keyword: mesh-electroporation
keyword: mesh-female
keyword: mesh-firefly-luciferin
keyword: mesh-gene-expression
keyword: mesh-genes-reporter
keyword: mesh-image-processing-computer-assisted
keyword: mesh-injections
keyword: mesh-injections-intraperitoneal
keyword: mesh-kinetics
keyword: mesh-knee-joint
keyword: mesh-luciferases-firefly
keyword: mesh-lumines
keyword: mesh-luminescent-agents
keyword: sdvbiolife-sciences-q-biobiotechnology
keyword: sdviblife-sciences-q-biobioengineering
issued: 2026-05-11T12:49:43.097839
modified: 2026-05-11T12:49:43.097845
language: en
contact point: Organization
  fn: CCSD

test_moissonnage_selune
type: Agent

Optical imaging of luminescence for in vivo quantification of gene electrotransfer in mouse muscle and knee.
type: Distribution
format: HTML
access URL: https://inserm.hal.science/inserm-00081253
issued: 2026-05-11T12:49:43.105586
modified: 2026-05-11T12:49:43.077311