Matrix metalloproteinases MMP-2, -9 and tissue inhibitors TIMP-1, -2 expression and secretion by primary human osteoblast cells in response to titanium, zirconia, and alumina ceramics.
type: Dataset
contact point: Organization
  fn: CCSD
keyword: mesh-humans
keyword: mesh-tissue-inhibitor-of-metallo
keyword: infoeu-reposemanticsarticle
keyword: mesh-osteoblasts
keyword: sdviblife-sciences-q-biobioengineering
keyword: mesh-dna-primers
keyword: journal-articles
keyword: mesh-gelatinase-b
keyword: mesh-gelatinase-a
keyword: mesh-tissue-inhibitor-of-metalloproteinase-1
keyword: mesh-base-sequence
keyword: mesh-enzyme-linked-immunosorbent-assay
keyword: mesh-aluminum-oxide
keyword: mesh-reverse-transcriptase-polymerase-chain-reaction
keyword: mesh-ceramics
landing page: 1549-3296
description: Osteogenic properties of bone cells are a key parameter governing osseointegration of implant devices. In this context, osteoblasts have a central role via extracellular matrix synthesis and remodeling that they regulate through different protease activity. In this study, we have analyzed the expression of two matrix metalloproteinases (MMPs): MMP-2 (72 kDa) and MMP-9 (92 kDa) and their specific tissue inhibitors TIMP-1 and TIMP-2 in primary human osteoblastic cells. The effect of titanium, zirconia, and alumina ceramics on the synthesis of these proteases was assessed using reverse transcriptase-polymerase chain reaction, enzyme-linked immunosorbent assay, and zymographic analysis. Our results showed that osteoblasts express MMP-2 and -9 mRNA. Furthermore, MMP-2 mRNA expression was decreased by titanium and increased by alumina whereas zirconia did not have any significant effect. Conversely, MMP-9 mRNA expression was stimulated by titanium but decreased with zirconia, whereas alumina induced no significant changes. Zymographic analysis has evidenced pro-MMP-2 gelatinolytic activity in all cell populations with time-dependent increase profile; pro-MMP-9, however, was not detected. Enzyme-linked immunosorbent assay data confirmed the production of MMP-2 and very low levels of MMP-9. In addition, TIMP-1 was secreted in 24-h-cultured cells and increased to maximal level at 48-72 h whereas TIMP-2 levels were very low. The interactions between human osteoblasts and the studied biomaterials altered both MMP-2, -9 and TIMP-1expression indicating that biomaterials may influence osseointegration and bone remodeling.
issued: 2026-05-15T01:18:45.822928
language: en
modified: 2026-05-15T01:18:45.822935

Matrix metalloproteinases MMP-2, -9 and tissue inhibitors TIMP-1, -2 expression and secretion by primary human osteoblast cells in response to titanium, zirconia, and alumina ceramics.
type: Distribution
access URL: https://inserm.hal.science/inserm-00077895
format: HTML
issued: 2026-05-15T01:18:45.851899
modified: 2026-05-15T01:18:45.761819

test_moissonnage_selune
type: Agent