@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-hal-00994823v1> a dcat:Dataset ;
    dct:description """
              We developed an image processing protocol to perform time lapse fluorescent analysis in microfluidic biochips. Using three probes to follow three parameters: the number of cell nucleus, the mitochondrial activity and the cell death, we have investigated the behaviour of HepG2/C3a under microfluidic culture conditions over a period of 10 h. The results have shown that cell response was independent of their location in the biochip, and cells aggregated after 4 h of perfusion whereas they spread in the Petri dish control. The mitochondrial activity increased with time in biochips when compared to Petri dishes. Nevertheless, the cell population presented mono-modal mitochondrial activity illustrating no specific cell subpopulation. The variance analysis of the parameters (via the interquartile calculation) revealed an increase in cell heterogeneity in the first 6 h to 7 h of cultures. Then the variance reached a plateau illustrating a stabilisation towards a homogeneous cell population in the biochips. Despite the fact that we also found a mono-modal cell population in Petri dishes, the variance depicted a continuous increase of the population heterogeneity. To highlight the potential of the image processing method for drug screening, we then performed a staurosporine toxicity assay. 5 μM treatments after 10 h of exposure led to 65% of cell death in the biochips whereas only 11% was detected in control. Altogether, these results illustrate the potential of our image processing technique combined with a microfluidic approach for wider real time cytotoxicity assays using on chip methods.
            """ ;
    dct:identifier "hal-00994823" ;
    dct:issued "2026-05-05T10:31:49.087102"^^xsd:dateTime ;
    dct:language "en" ;
    dct:modified "2026-05-05T10:31:49.087106"^^xsd:dateTime ;
    dct:publisher <https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> ;
    dct:title "Characterisation of early HepG2/3a cell response to a microfluidic culture in liver biochips using multi-parametric real time image processing" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "CCSD" ] ;
    dcat:distribution <https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-hal-00994823v1/resource/756aed2f-712f-4d65-9d43-be06236bffac> ;
    dcat:keyword "fluorescent-multi-parametric-analysis",
        "hepg2c3a",
        "image-processing",
        "infoeu-reposemanticsarticle",
        "journal-articles",
        "microfluidic-biochips",
        "mitochondrial-activity",
        "staurosporine" ;
    dcat:landingPage <ISSN:%200925-4005> .

<ISSN:%200925-4005> a foaf:Document .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-hal-00994823v1/resource/756aed2f-712f-4d65-9d43-be06236bffac> a dcat:Distribution ;
    dct:format "HTML" ;
    dct:issued "2026-05-05T10:31:49.088698"^^xsd:dateTime ;
    dct:modified "2026-05-05T10:31:49.076723"^^xsd:dateTime ;
    dct:title "Characterisation of early HepG2/3a cell response to a microfluidic culture in liver biochips using multi-parametric real time image processing" ;
    dcat:accessURL <https://hal.science/hal-00994823> .

<https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> a foaf:Agent ;
    foaf:name "test_moissonnage_selune" .