Wheat centromeric retrotransposons: the new ones take a major role in centromeric structure
type: Dataset
description: The physical map of the hexaploid wheat chromosome3B was screened using centromeric DNA probes. A 1.1-Mb region showing the highest number of positive bacterial artificial chromosome (BAC) clones was fully sequenced and annotated, revealing that 96% of the DNA consisted of transposable elements, mainly long terminal repeat (LTR) retrotransposons (88%). Estimation of the insertion time of the transposable elements revealed that CRW (also called Cereba) and Quinta are the youngest elements at the centromeres of common wheat (Triticumspp.) and its diploid ancestors, with Quinta being younger than CRW in both diploid and hexaploid wheats. Chromatin immunoprecipitation experiments revealed that both CRW and Quinta families are targeted by the centromere-specific histone H3 variant CENH3. Immuno colocalization of retroelements and CENH3 antibody indicated that a higher proportion of Quinta than CRWs was associated with CENH3, although CRWs were more abundant. Long arrays of satellite repeats were also identified in the wheat centromere regions, but they lost the ability to bind with CENH3. In addition to transposons, two functional genes and one pseudogene were identified. The gene density in the centromere appeared to be between three and four times lower than the average gene density of chromosome3B. Comparisons with related grasses also indicated a loss of microcollinearity in this region. Finally, comparison of centromeric sequences of Aegilops tauschii (DD), Triticum boeoticum (AA) and hexaploid wheat revealed that the centromeres in both the polyploids and diploids are still undergoing dynamic changes, and that the new CRWs and Quintas may have undertaken the core role in kinetochore formation.
issued: 2026-05-05T21:35:07.226161
language: en
modified: 2026-05-05T21:35:07.226167
contact point: Organization
  fn: CCSD
keyword: cenh3
keyword: centromere
keyword: chromosome-3b
keyword: evolution
keyword: infoeu-reposemanticsarticle
keyword: journal-articles
keyword: retrotransposon
keyword: sdvsalife-sciences-q-bioagricultural-sciences
keyword: triticum-aestivum
landing page: 0960-7412

Wheat centromeric retrotransposons: the new ones take a major role in centromeric structure
type: Distribution
format: HTML
issued: 2026-05-05T21:35:07.240387
modified: 2026-05-05T21:35:07.209254
access URL: https://hal.science/hal-00964447

test_moissonnage_selune
type: Agent